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Plasmid#187104PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVP65KR-gb-SacB(-)
Plasmid#171646PurposeEquipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.DepositorInsertMBP-mCherry-8xHis-SacB(-)
UseTags8xHisExpressionMutationinternal HindIII site in the SacB gene was remove…PromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS*11-yfp0
Plasmid#53241Purposeexpress yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPLtetO1Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDC2.1.0-gDNA
Plasmid#113781PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2DepositorInsertCSDC2 (CSDC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry
Plasmid#223652PurposeExpresses hM3D(Gq) in a flippase dependent mannerDepositorInserthM3D(Gq)-mCherry
UseAAVTagsmCherryExpressionMutationPromoterhSynAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
hPDZD11-HA
Plasmid#236537PurposeFor expression of PDZD11 (human) in mammalian cellsDepositorInsertPDZD11 (PDZD11 Human)
UseTagsHAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-OXER1-mKate2
Plasmid#226713Purposeoverexpression red fluorescent OXER1 protein in zebrafish larvaeDepositorInsertOXER1 (OXER1 Human)
UseUnspecified; Zebrafish expressionTagsmKate2ExpressionMutationPromoterSP6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)
Plasmid#225430PurposeOCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-FRT-OCSterm-FRT-miniDFR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4652
Plasmid#215266PurposeTU for the copper regulated expression of the flippase (FLP) recombinase.DepositorInsertCBS:miniDFR:FLP:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
Plasmid#125797PurposeHistone H2B-superfolder GFP and tdTomato fused by unskippable linkerDepositorInsertH2BsfGFP-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOZ-FH-C Bach1 K141A/K142A
Plasmid#73646PurposeRetroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)DepositorInsertBach1 (BACH1 Human)
UseRetroviralTagsFLAG and HisExpressionMammalianMutationK141A/K142A ( mlh1 binding mutant)PromoterunknownAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Flp-2A-BleoR
Plasmid#232752PurposeIVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance geneDepositorInsertsFlpO
BleoR
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH2.12_GagZip-MCP-P2A-eGFP
Plasmid#205527PurposeExpress HIV GagZip-MCP-P2A-eGFPDepositorInsertGagZip-MCP
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mKate2
Plasmid#104009PurposePlasmid encoding mKate2DepositorInsertmKate2
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, V45A, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL wt-pBabe-puro
Plasmid#19234DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwtPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPIPL12-FnCas12a
Plasmid#199563PurposeTetracycline-Inducible Fncas12a gene expression in E. coli and clostridiaDepositorInsertFncas12a
UseCRISPR and Synthetic Biology; Tetracycline induci…TagsExpressionBacterialMutationPromoterCOtetR-PIPL12Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express2
Plasmid#103999PurposePlasmid encoding DsRed-Express2DepositorInsertDsRed-Express2
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCherry
Plasmid#99265PurposeContains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmCherry
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 beta1
Plasmid#16042DepositorInsertbeta1 (ITGB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
DR274-eGFP sgRNA
Plasmid#61051Purposefor generation of a eGFP specific sgRNA from a T7 promoterDepositorInsertEGFP sgRNA
UseCRISPRTagsExpressionMutationPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
Plasmid#74010PurposeTissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoterDepositorInserts5xUAS
Cas9
Cre
U6 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP-T
Plasmid#103998PurposePlasmid encoding TagRFP-TDepositorInsertTagRFP-T
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB7 alpha5
Plasmid#16041DepositorInsertalpha5 (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP
Plasmid#103997PurposePlasmid encoding TagRFPDepositorInsertTagRFP
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only