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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,529 results for: golden gate

Showing: 1081 - 1100 of 3529 results

  1. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

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  2. pKS1429

    Plasmid
    #239514
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239514-plasmid-map-sequence-id-478375

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  3. pKS1173

    Plasmid
    #239507
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239507-plasmid-map-sequence-id-478369

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  4. pKS1334

    Plasmid
    #239508
    Purpose
    Express CDT2 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT2-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    239508-plasmid-map-sequence-id-477192

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  5. pKS1140

    Plasmid
    #239501
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239501-plasmid-map-sequence-id-475913

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  6. pKS1577

    Plasmid
    #239525
    Purpose
    Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScVba2p-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239525-plasmid-map-sequence-id-475912

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  7. pKS1141

    Plasmid
    #239502
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239502-plasmid-map-sequence-id-475904

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  8. pKS1461

    Plasmid
    #239515
    Purpose
    Express the lower portion of melvaonate pathway enzymes (Erg12p, Erg8p, and Erg19p) in the peroxisome; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScErg12p-ePTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239515-plasmid-map-sequence-id-475897

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  9. pKS1172

    Plasmid
    #239506
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239506-plasmid-map-sequence-id-475884

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  10. pKS1428

    Plasmid
    #239513
    Purpose
    Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    AtUTR1-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239513-plasmid-map-sequence-id-475875

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  11. pVL1280

    Plasmid
    #239539
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239539-plasmid-map-sequence-id-475864

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  12. pAIP4_2RH3

    Plasmid
    #234219
    Purpose
    Heterologous protein expression of protein virC2 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2RH3 (virC2 Agrobacterium tumefaciens)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234219-plasmid-map-sequence-id-464184

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  13. pAIP4_5Y33

    Plasmid
    #234204
    Purpose
    Heterologous protein expression of alginate lyase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5Y33
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234204-plasmid-map-sequence-id-464169

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  14. pAIP4_4REH

    Plasmid
    #234195
    Purpose
    Heterologous protein expression of major latex-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4REH
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234195-plasmid-map-sequence-id-464160

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  15. pAIP4_4ZHW

    Plasmid
    #234158
    Purpose
    Heterologous protein expression of YfiB in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ZHW (yfiB Pseudomonas aeruginosa PAO1)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234158-plasmid-map-sequence-id-464124

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  16. pAIP4_1XSZ

    Plasmid
    #234151
    Purpose
    Heterologous protein expression of RalF in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1XSZ (ralF Legionella pneumophila)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234151-plasmid-map-sequence-id-464117

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  17. pAIP4_2D5J

    Plasmid
    #234143
    Purpose
    Heterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2D5J
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234143-plasmid-map-sequence-id-464109

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  18. L1B39 - pMUTE-Bxb1-tUBQ10

    Plasmid
    #219704
    Purpose
    Level 1 pMUTE-Bxb1-tUBQ10, for stomata specific expression of Bxb1 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pMUTE:Bxb1:tUBQ10
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219704-plasmid-map-sequence-id-435734

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  19. PROM_10

    Plasmid
    #201301
    Purpose
    D10: Promoter of TraesCS3D02G517600 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D10: Promoter of TraesCS3D02G517600
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201301-plasmid-map-sequence-id-410981

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  20. pLM001

    Plasmid
    #201311
    Purpose
    AvrSr50 (with stop codon) as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr50
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    201311-plasmid-map-sequence-id-410106

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Showing: 1081 - 1100 of 3529 results