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Plasmid#219714PurposeLevel 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG5-tUBQ10-GG6
UseSynthetic BiologyTagsExpressionMutationWT terminator with added golden gate linkersPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T13 GG3-tRBCS-mCherry-NLS-GG4
Plasmid#219696PurposeLevel 0 to construct history-dependent targetDepositorInsertGG3-tRBCS-mCherry-NLS-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1
Plasmid#219697PurposeLevel 0 to construct history-dependent targetDepositorInsertGG0-tUBQ1-NLS-NeonGreen-GG1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6
Plasmid#219693PurposeLevel 0 to construct history-dependent target T2PBDepositorInsertGG4-Bxb1B-PhiC31B-GG6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I80_Bxb1mut(noBsaI)_GG1-GG4
Plasmid#219715PurposeLevel 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.DepositorInsertGG1-Bxb1(noBsaI0)-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/κ
Plasmid#52213PurposeHuman immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/λ
Plasmid#52214PurposeHuman immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin lambda constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_1_001
Plasmid#167347PurposeddPCR gating control for droplets containing all 5 targets (2 in pol)DepositorInsertpol_gag_tat_env
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_9_003
Plasmid#167349PurposeddPCR gating control for droplets containing either gag+env targets OR tat + env targetsDepositorInsertgag_tat_env
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_6_002
Plasmid#167348PurposeddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targetsDepositorInsertpol_gag_tat
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_29_006
Plasmid#167352PurposeddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targetsDepositorInsertpol_env
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_14_004
Plasmid#167350PurposeddPCR gating control for droplets containing the env targetDepositorInsertenv
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_32_007
Plasmid#167353PurposeddPCR gating control for droplets containing 5'pol or 3'pol targetsDepositorInsertpol
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_17_005
Plasmid#167351PurposeddPCR gating control for droplets containing gat and tat targetsDepositorInsertgat_tat
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-IRES_ECFP
Plasmid#48354PurposeContributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.DepositorInsertIRES_ECFP
UseGateway entry vectorTagsExpressionMutationECFP translation is mediated by an IRES. Contains…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLXIN2-RFP
Plasmid#99205PurposeRFP expressing bicistronic retroviral vectorDepositorInsertRed fluorescent protein
UseRetroviral; Bicistronic retroviral expression vec…TagsExpressionMammalianMutationThe dsRed cDNA was PCR amplified off AddGene plas…PromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6
Plasmid#219712PurposeLevel 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.DepositorInsertGG4-pGATA23-Bxb1-tUBQ10-GG6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T7 Circuit 2 GG1-PhiC31P-bbs1-BxB1P-GG2
Plasmid#219692PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG1-PhiC31P-bbs1-BxB1P-GG2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I89-pMUTE_GG0-1
Plasmid#219708PurposeLevel 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pMUTE-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I74-_tUBQ10_GG4-GG6
Plasmid#219713PurposeLevel 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG4-tUBQ10-GG6
UseSynthetic BiologyTagsExpressionMutationWT terminator with added golden gate linkersPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only