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We narrowed to 4,839 results for: pcr

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Showing: 1081 - 1100 of 4839 results

  1. pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

    Plasmid
    #240799
    Purpose
    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem
    Depositor
    Hans Clevers
    Publication
    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeonGreen-mCherry-CAAX
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    none
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240799-plasmid-map-sequence-id-481917

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  2. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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  4. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

    Plasmid
    #46839
    Purpose
    Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated
    Depositor
    Phil Sharp
    Publication
    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    46839-plasmid-map-sequence-id-66684

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  6. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid
    #140200
    Purpose
    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140200-plasmid-map-sequence-id-310179

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  7. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid
    #140204
    Purpose
    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Step 1 of cloning c…
    Promoter
    Aspergillus fumigatus U3 (RNAPIII).
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    140204-plasmid-map-sequence-id-309884

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  9. pCru5-GGG/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49221
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49221-plasmid-map-sequence-id-73631

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  10. pCru5-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49223
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49223-plasmid-map-sequence-id-73635

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  19. pRS414-Syf1

    Plasmid
    #234114
    Purpose
    Syf1 region PCR, restriction digest and ligation into pRS414 backbone
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234114-plasmid-map-sequence-id-465994

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  20. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61973-plasmid-map-sequence-id-101284

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Showing: 1081 - 1100 of 4839 results