We narrowed to 11,807 results for: ARIA

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pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

Plasmid

#111393

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

Plasmid

#111388

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
ChETA

Use

AAV

Tags

HA and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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p2R3a-VenusYFP-3AT

Plasmid

#71269

Purpose

Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

VenusYFP

Use

Gateway

Tags

4xGly linker and T3A pea Pisum sativum ribulose-1…

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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plx307 SHOC2 V5 WT

Plasmid

#190568

Purpose

SHOC2 variant overexpression

Depositor

William Hahn

Article

Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTH733-2µ-RLuc/maxCFLuc

Plasmid

#40608

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT K842M

Plasmid

#154284

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

K842M mutation (Catalytic Inactive)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v1 IQSEC1 WT

Plasmid

#162016

Purpose

Expression of IQSEC1 splice variant 1

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

Plasmid

#212487

Purpose

Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v2 IQSEC1 WT

Plasmid

#162017

Purpose

Expression of IQSEC1 splice variant 2

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pαH-S-HRV3C

Plasmid

#164567

Purpose

SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike - Furin site changed for HRV3C site

Tags

2X Strep-Tag II and 8X His tag

Expression

Mammalian

Mutation

682-685 (furin site) replaced with HRV3C site (LE…

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-V5H6-mPCSK3 D153A

Plasmid

#122681

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Mutation

changed Aspartic Acid 153 to Alanine

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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LIC1B_Caspase-LOVN7C-7 C450A

Plasmid

#104628

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Mutation

C450A (dark-locked and light-insensitive mutation)

Promoter

T7

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

Plasmid

#69051

Purpose

lentiviral expression of IKZF1 with HA tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

splice variant 2

Promoter

UBC

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Article

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v3 IQSEC1 WT

Plasmid

#162018

Purpose

Expression of IQSEC1 splice variant 3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v4 IQSEC1 WT

Plasmid

#162019

Purpose

Expression of IQSEC1 splice variant 4

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A mKate2-hu_ncOGT D554N

Plasmid

#154283

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

D554N mutation (Ser/Thr Glycosylation Impaired)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-actin-pcDNA4-TO

Plasmid

#118370

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Tags

HA-GFP1-10

Expression

Mammalian

Promoter

CMV/TO

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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3322_pETcon_SARS2_Omicron-BA2

Plasmid

#184409

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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