We narrowed to 17,551 results for: emb

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pUC18 3xFLAG COP1

Plasmid

#237334

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

COP1 (COP1 Human)

Expression

Bacterial

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG FBXO15

Plasmid

#236456

Purpose

transient overexpression of FBXO15 in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

FBXO15 (FBXO15 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-LOV2-OMP25

Plasmid

#233346

Purpose

Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted and N538E

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_D-Myc

Plasmid

#236525

Purpose

For expression of Chimera of B-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_D-Myc

Plasmid

#236523

Purpose

For expression of Chimera of D-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-cCGNL1_B-Myc

Plasmid

#236522

Purpose

For expression of Chimera of B-region cCGNL1 in cCGN in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGN-CGNL1 (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-cCGN_B-Myc

Plasmid

#236524

Purpose

For expression of Chimera of D-region cCGN in cCGNL1 in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al J Biol Chem. 2012 Apr 13;287(16):13159-6

Insert

CGNL1-CGN (CGN Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSMPPv2-Tau-RING (M72E/I18R)

Plasmid

#233690

Purpose

Expression of Tau-RING I18R-M72E mutant in mammalian cells

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Tau protein fused to TRIM21 RING domain carrying M72E/I18R mutations

Use

Lentiviral

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pOPTG-Tau-RING (M72E/I18R)

Plasmid

#233692

Purpose

Expression of Tau-RING I18R-M72E mutant in bacterial cells

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Tau protein fused to TRIM21 RING domain

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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STp6

Plasmid

#235740

Purpose

Protein expression of human RAB5A Q79,C19S,C63S,S84TAG, 1-212-His6 in bacteria for unnatural amino acid incorporation

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5A (RAB5A Human)

Tags

6xHis

Expression

Bacterial

Mutation

Q79, C19S, C63S, S84TAG, aa 1-212

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJB78

Plasmid

#235738

Purpose

Protein expression of GST-TEV-HsRAB1A Q70L C26S C126S in bacteria for maleimide conjugation

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB1A (RAB1A Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

Q70L C26S C126S, aa 1-204

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[G249D]-P2A-Electra1 (pAH10)

Plasmid

#227615

Purpose

Overexpression of SLC25A46 with G249D mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

G249D

Promoter

CMV, T7

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP1-mScarleti

Plasmid

#228526

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Puro-DEST (JDW 925)

Plasmid

#229808

Purpose

A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGLAST-DEST (JDW 1009)

Plasmid

#229822

Purpose

A single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGM63

Plasmid

#220169

Purpose

CRISRPi guide RNA against TOMM70A

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM70A (sgRNA) (TOMM70 Human)

Use

Lentiviral

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attL1/L2 flanked MCS

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-StayGoldc4-Giantin (JDW 1355)

Plasmid

#229844

Purpose

A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

n2StayGoldc4

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAG391

Plasmid

#226732

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

GFP11

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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