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TRS6E1b-luc(-732/-721) mutant1

Plasmid

#45384

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI1 promoter -732/-721 mutated at -732/-731

Use

Luciferase

Expression

Mammalian

Mutation

mutated -732/-731 from ag to tc

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-H122Y

Plasmid

#33085

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H122Y

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pLN428 (FuGW-G5p-mKate2-1Pe )

Plasmid

#105184

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pLN434 (FuGW-G14p-mKate2-1Pe )

Plasmid

#105188

Purpose

Module 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G14p-mKate2-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEB2-mRuby3 Addgene

Plasmid

#104005

Purpose

Plasmid encoding mRuby3 Addgene

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRuby3 Addgene

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express2

Plasmid

#103999

Purpose

Plasmid encoding DsRed-Express2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut

Plasmid

#42045

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(925-1709)

Plasmid

#42004

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa925-1709

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(1-924)

Plasmid

#42003

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1-924

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(1-1223)

Plasmid

#42013

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1-1223

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(1-1076)

Plasmid

#42014

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1-1076

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-EGFP

Plasmid

#175827

Purpose

Express EGFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab7

Plasmid

#174458

Purpose

Express mCherry with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hPMLIII

Plasmid

#103807

Purpose

BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hPMLIII (CIB1, PML Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hLaminB1

Plasmid

#103799

Purpose

BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hLaminB1 (CIB1, LMNB1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-dL5-2XG4S-actin

Plasmid

#73262

Purpose

Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

Insert

KozATG-dL5-2XG4S-actin (ACTB Synthetic, Human)

Tags

The FAP and actin are fused with 2 copies of a G4…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox1

Plasmid

#50778

Purpose

retroviral expression of mouse Sox1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox1 (Sox1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP in Artichoke

Plasmid

#169931

Purpose

Overexpression of Full Length of BCL6-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 (BCL6 Human)

Use

Lentiviral

Tags

GFP

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-TagBFP-W

Plasmid

#126687

Purpose

Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

TagBFP

Use

Lentiviral

Promoter

Ef1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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