We narrowed to 66,059 results for: nin
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CRISPRoff-mScarletI
Plasmid#217783PurposeExpresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencingDepositorInsertCRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
Tags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletIExpressionMammalianPromoterCAGAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
TagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-Nir1-LNS2
Plasmid#220084Purposehigh affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)DepositorInsertPITPNM3 (PITPNM3 Human)
TagsmCherry-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (V155M)
Plasmid#214161PurposeTransient expression of gain-of-function STING mutantDepositorAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSOX9-dTAG-mNeonGreen-V5
Plasmid#194971PurposeAAV vector for knocking in a C-terminal tag (dTAG-mNeonGreen-V5) for human SOX9DepositorAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR
Plasmid#181973PurposeExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-BBz CAR; LTBR (LTBR Synthetic, Human)
UseLentiviralAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2_SEPT6
Plasmid#174497Purposebacterial co-expression of human SEPT2 and of human SEPT6DepositorAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti SYN-SVI-DIO-dCas9-VPR
Plasmid#164576PurposeExpresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.DepositorInsertA DIO FLAG-dCas9-VPR cassette containing an SV40 intron
UseCRISPR, Cre/Lox, and LentiviralTagsFLAGExpressionMammalianAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
INS-2A-luciferase-2A-CD19-GFP
Plasmid#159349PurposeHuman INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.DepositorInsertsUseIns locus knockin donor vectorAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4591NES
Plasmid#138377PurposeBooster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.DepositorInsertBooster-ROCK
ExpressionMammalianPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:GrRenilla
Plasmid#118067PurposeTranscriptional unit for constitutive expression of GrRenilla in mammalian cellsDepositorInsertCMV:GreenRenilla:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRedF
Plasmid#118057PurposeGBPart - RedF Luciferase CDSDepositorInsertRed Firefly Luciferase CDS
UseSynthetic Biology; Domestication of dna parts for…MutationRed mutant S286YAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)
Plasmid#79619PurposeExpresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorPublicationInsertSpCas9
Tags3xFlag-PmCDA1ExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
hSERT-N1-pECFP
Plasmid#70106Purposemammalian expression of hSERT with C-terminal CFP fusionDepositorAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Mev1
Plasmid#65815PurposeNADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli ExpressionDepositorInsertsmvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
mvaS: hydroxymethylglutaryl-CoA synthase
ExpressionBacterialPromoterinsulated mipAp from E. coli and insulated waaHp…Available sinceSept. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE
Plasmid#67295PurposeInduce human iN conversion from fibroblastDepositorAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
fck-Jaws-KGC-GFP-ER2
Plasmid#65012PurposeLentivirus-mediated expression of Jaws under the CaMKII promoter.DepositorInsertJaws-KGC-GFP-ER2
UseLentiviralTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR1A-Yap S127A
Plasmid#46050DepositorInsertYes-kinase associated protein (YAP1 Human)
UseGateway CloningTags2X Flag tagMutationS127AAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO GFP HSPA14
Plasmid#19488DepositorAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only
