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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(WT)

Plasmid

#86637

Purpose

Expresses eGFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-p53-shRNA-941

Plasmid

#25637

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-941 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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HA-TAZ-S89A

Plasmid

#32840

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Tags

HA

Expression

Mammalian

Mutation

S89A

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE-GFP

Plasmid

#106281

Purpose

A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6/UbC/mSlc7a1

Plasmid

#17224

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Slc7a1 (Slc7a1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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PB_iMYOD1_P2A_GFP_Puro

Plasmid

#168804

Purpose

PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

MYOD1 (MYOD1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-p53-shRNA-427

Plasmid

#25636

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-427 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-IRES2-mCherry

Plasmid

#159600

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-IRES2-mCherry

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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DD-DamWT-IRES2-mCherry

Plasmid

#159602

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the wild-type allele of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamWT-IRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Claspin

Plasmid

#12659

Depositor

Michele Pagano

Article

Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

Insert

Claspin (CLSPN Human)

Tags

Flag

Expression

Mammalian

Available Since

May 16, 2007

Availability

Academic Institutions and Nonprofits only
pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-Ufm1 ∆C3

Plasmid

#133866

Purpose

Expression of human UFM1 mutant lacking the last three C-terminal residues

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hFIP200

Plasmid

#24300

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

FIP200 (RB1CC1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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