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pDest-669 (attR4-attR3)

Plasmid

#161887

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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peft-3::cas-9::tbb-2 3'UTR

Plasmid

#48960

Purpose

this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Insert

cas9

Use

CRISPR

Promoter

eef-1A.1 (eft-3)

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJA255

Plasmid

#21508

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5::citrine (w introns)::(Gly)5Ala

Use

Multisite gateway

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pMH-02

Plasmid

#158432

Purpose

pBAD33 vector encoding MotA, eGFP-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

eGFP

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJJB170

Plasmid

#158600

Purpose

Transcription factors ADR1c, PIP2c, OAF1c

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Inserts

ADR1c
PIP2c
OAF1c

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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4A3.SU

Plasmid

#197675

Purpose

Expresses P1 S tail fibre and U chaperone

Depositor

Baojun Wang

Article

Huan et al J Mol Biol. 2022 Sep 15;434(21):167829.

Inserts

S
U

Expression

Bacterial

Promoter

Late S promoter, LPS and N/A

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDM767

Plasmid

#207425

Purpose

Extrachromosomal expression HSPC300-GFP and PH-CRAC-mRFPmars

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
CRAC

Use

Unspecified

Tags

GFP and mRFPmars

Mutation

aa 1–126 (PH domain) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDM1424

Plasmid

#207424

Purpose

Extrachromosomal expression HSPC300-GFP and PkgE-PH-mCherry

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
PkgE

Use

Unspecified

Tags

GFP and mCherry

Mutation

aa 1–100 (PH domain) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL658

Plasmid

#167837

Purpose

Encodes OptoLAC1B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (LAA)

Expression

Bacterial

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL659

Plasmid

#167838

Purpose

Encodes OptoLAC2B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (AAV)

Expression

Bacterial

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAB147

Plasmid

#101662

Purpose

Opto-T7RNAP*(302)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(302)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMH-03

Plasmid

#158433

Purpose

pBAD33 vector encoding MotA, yPET-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

yPET

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pM9_NR_HD

Plasmid

#90415

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_HD

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJA258

Plasmid

#21511

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR

Use

Multisite gateway

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Hec1 mCherry Plk1 T210D

Plasmid

#45205

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted mCherry Plk1 T210D

Tags

mCherry

Expression

Mammalian

Mutation

Plk1 T210D mutation

Promoter

T7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pNXRVj-HACre

Plasmid

#78407

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
aur1R (aur1 Fission Yeast)

Expression

Yeast

Mutation

Gly 240 to Cys

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDest-312 (attR4-attR3)

Plasmid

#161897

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-302 (attR4-attR2)

Plasmid

#161894

Purpose

Multisite Gateway (attR4-attR2) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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