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MTK3_038

Plasmid

#123752

Purpose

Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

R-FlincA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Tags

DsbC-6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L

Plasmid

#182279

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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pET28-2H12

Plasmid

#221186

Purpose

His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2

Plasmid

#14129

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pEHlyA5

Plasmid

#168298

Purpose

Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

Depositor

Luis Ángel Fernández

Article

Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

Insert

N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA

Tags

E-tag, HA-tag, His-tag, Prescission site, and VHH…

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-gRNA-Actb1216

Plasmid

#155368

Purpose

PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

PspCas13b gRNA targeting Actb 1216

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Ser)

Plasmid

#109352

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Changed Cys at position 391 to Serine (Pertussis …

Promoter

CAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR

Plasmid

#112159

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

Plasmid

#204497

Purpose

Expresses rtTA and Neomycin resistance genes in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

rtTA

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PylRS-AS

Plasmid

#137908

Purpose

For incorporation of fluorophenylalanine derivatives

Depositor

Wenshe Liu

Article

Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

Containing N346A, C348S mutations

Promoter

pBAD

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX DAB1

Plasmid

#30139

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Disabled-1 (Dab1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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mNeongreen-2xrGBD

Plasmid

#129624

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

mNeongreen-2xrGBD

Expression

Mammalian

Promoter

CMVdel

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125691

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTagRFP-C1-Fibrillarin

Plasmid

#70649

Purpose

Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

Fibrillarin (FBL Human)

Tags

TagRFP

Expression

Mammalian

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_WT

Plasmid

#79010

Purpose

For mammalian expression of WT AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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