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psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

Plasmid

#61796

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

Plasmid

#61793

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Mutation

miR-200c MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

Plasmid

#61791

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

Plasmid

#61790

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

Plasmid

#61786

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLexA::SRDX (GB0732)

Plasmid

#68184

Purpose

Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LexA Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSR11

Plasmid

#69154

Purpose

read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
fzr-1 (fzr-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

fzr-1 and rps-27

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pES006 (pTet-qacR 2/pQacA-deGFP)

Plasmid

#69035

Purpose

reporter with qacR 2 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pQacA and pTet

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGal4BD::BDR (GB0734)

Plasmid

#68185

Purpose

Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Gal4 Binding Domain fused to BDR Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHL836

Plasmid

#62827

Purpose

Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

gfp
ipex leader
hfq leader

Use

Synthetic Biology

Tags

ompC leader and st7 RBS

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHL2107

Plasmid

#62841

Purpose

Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

m8::micC leader
T710 hfq leader
gfp

Use

Synthetic Biology

Tags

m9::ompC leader

Expression

Bacterial

Promoter

pConNoHind, pLlacO-1, and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
CFP(159-238) in pcDNAI/Amp

Plasmid

#60896

Purpose

This vector attaches CFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(159-238)

Expression

Mammalian

Mutation

Asn-164 to His

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT

Plasmid

#61794

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Snail1-3'UTR-MRE-1 MUT

Plasmid

#61795

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT

Plasmid

#61797

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

Plasmid

#61787

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR with miR-200c MRE-2 mutated

Use

Luciferase

Mutation

miR-200c MRE-2 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

Plasmid

#61788

Purpose

Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Tob1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc Separase delta 55

Plasmid

#59824

Purpose

Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 55

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-hRIP1-FL I541P

Plasmid

#61381

Purpose

Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP1with I541P mutation (RIPK1 Human)

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

changed I541 to P

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only