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pCAGEN_NES_V5_eMagBF_N73

Plasmid

#235635

Purpose

Encodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

NES-V5-eMagBF

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N65_eMagBF_V5_NES

Plasmid

#235612

Purpose

Encodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-65

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N77_eMagBF_V5_NES

Plasmid

#235616

Purpose

Encodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-77

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N99_eMagBF_V5_NES

Plasmid

#235618

Purpose

Encodes ALIBi N-terminal fragment, residues 1-99, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-99

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.3-Dadh-ΔElen0473(ElA2)

Plasmid

#225318

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0473) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0473)

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.6-Dadh-ΔElen0476(ElA2)

Plasmid

#225321

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0476) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0476)

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.1-Dadh-ΔElen0469(ElA2)

Plasmid

#225315

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0469)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.4-Dadh-ΔElen0474(ElA2)

Plasmid

#225319

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0474)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)

Plasmid

#225322

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0477)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.8-Dadh-ΔElen0478(ElA2)

Plasmid

#225323

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0478)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.9-Dadh-ΔElen0481(ElA2)

Plasmid

#225324

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0481)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.10-Dadh-ΔElen0482(ElA2)

Plasmid

#225325

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0482)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_3EYE

Plasmid

#234176

Purpose

Heterologous protein expression of N-acetylgalactosamine-specific enzyme IIB component of PTS in Escherichia coli

Depositor

Christopher Bahl

Insert

3EYE

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pJJB1462

Plasmid

#218623

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1463

Plasmid

#218624

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9A-HA

Plasmid

#209230

Purpose

Mammalian expression of ATP9A

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9A (ATP9A Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-7

Plasmid

#220464

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Tags

3xFLAG

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-7.M4

Plasmid

#220465

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Tags

3xFLAG

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-p31(comet)

Plasmid

#221697

Purpose

Mammalian expression of HA-tagged human p31(comet)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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