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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSM045

Plasmid

#217399

Purpose

KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE1

Expression

Bacterial

Promoter

PTrc

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSM045

Plasmid

#217399

Purpose

KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE1

Expression

Bacterial

Promoter

PTrc

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1464

Plasmid

#213736

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1464

Plasmid

#213736

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1481

Plasmid

#213807

Purpose

LguI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

LguI-associated switch methylase M.XmnI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1481

Plasmid

#213807

Purpose

LguI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

LguI-associated switch methylase M.XmnI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1474

Plasmid

#213762

Purpose

BpiI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated switch methylase M2.NmeMC58II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1474

Plasmid

#213762

Purpose

BpiI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated switch methylase M2.NmeMC58II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1488

Plasmid

#213813

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.MboII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1488

Plasmid

#213813

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.MboII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1479

Plasmid

#213805

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1479

Plasmid

#213805

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1480

Plasmid

#213806

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1480

Plasmid

#213806

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge