Addgene

We narrowed to 4,952 results for: puc

Showing: 2751 - 2800 of 4952 results

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pART (pT-2)

Plasmid

#62708

Purpose

Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEPQDKN0248

Plasmid

#162313

Purpose

Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-sfGFP-HiBiT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCrAb-km

Plasmid

#214199

Purpose

Expresses crRNA for genome editing in Acinetobacter baumannii

Depositor

Yu Wang

Article

Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

Insert

crRNA-HDV

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23119

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_N_allele_400

Plasmid

#161767

Purpose

Donor vector for endogenous tagging of human FUS at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGGA004

Plasmid

#48815

Purpose

Provides the 35S promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

35S promoter

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition site removed by substit…

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.Y

Plasmid

#201004

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pESR1-donor

Plasmid

#112337

Purpose

CRISPR donor plasmid to tag human transcription factor ESR1 with GFP

Depositor

Kevin White

Insert

ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)

Use

CRISPR

Tags

Expression

Mutation

Unknown

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFOXF2-donor

Plasmid

#113816

Purpose

CRISPR donor plasmid to tag human transcription factor FOXF2 with GFP

Depositor

Kevin White

Insert

FOXF2 homology arms flanking EGFP-IRES-Neo cassette (FOXF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMIN-0

Plasmid

#197782

Purpose

Level 2 destination vector: minimal (non-T-DNA) backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_C_allele_400

Plasmid

#161768

Purpose

Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> :dTomato:IRES:Puro

Plasmid

#110832

Purpose

Expresses dTomato (dT) red fluorescent reporter.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

dTomato Tag

Use

Non-viral gene expression vector

Tags

IRES

Expression

Mutation

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Use

Tags

3xFlag and myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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LI D-E mOrange + link c2

Plasmid

#54421

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + mOrange

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LRRK2_Halo_N_allele

Plasmid

#178142

Purpose

Donor vector for endogenous tagging of human LRRK2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (LRRK2 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGGF001

Plasmid

#48842

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::BastaR::tMAS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0285

Plasmid

#162290

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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LI D-E HA

Plasmid

#54449

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C HA

Plasmid

#54448

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pERF-donor

Plasmid

#112299

Purpose

CRISPR donor plasmid to tag human transcription factor ERF with GFP

Depositor

Kevin White

Insert

ERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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LI E-F HSP-T

Plasmid

#54432

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Heat Shock Protein terminator A.thaliana

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI F-G neo c2

Plasmid

#54405

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

neomycin phosphotransferase II (nptII)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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TC550

Plasmid

#164891

Purpose

Punc-4::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

unc4-rtTA(Q)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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LI D-E Cerulean + linker c5

Plasmid

#54413

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + CFP Cerulean

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pISL1-donor

Plasmid

#113820

Purpose

CRISPR donor plasmid to tag human transcription factor ISL1 with GFP

Depositor

Kevin White

Insert

ISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Unknown

Promoter

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CACNA1A_Halo_N_allele

Plasmid

#178152

Purpose

Donor vector for endogenous tagging of human CACNA1A at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (CACNA1A Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AP682-1

Plasmid

#70050

Purpose

Expresses TEV::eGFP::myc::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

eGFP

Use

Tags

TEV and myc::3Xflag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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LI D-E 6xHIS

Plasmid

#54447

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

polyhistidine (6x) tag

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E C-NLS c2

Plasmid

#54428

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

NLS of SV40 large T antigen

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI E-F nos-T c2

Plasmid

#54407

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nopaline synthase terminator

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI B-C mOrange

Plasmid

#54427

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mOrange + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVC_CRISPR_Cat

Plasmid

#120368

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR_Cat

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LI B-C T-Saphire

Plasmid

#54424

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

T-saphhire + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI D-E T-Saph + linker c1

Plasmid

#54412

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + T-Sapphire

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI B-C Cerulean

Plasmid

#54425

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP-Cerulean + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI B-C YFP

Plasmid

#54423

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

YFP-Venus + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI D-E YFP + linker c1

Plasmid

#54411

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + YFP Venus

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI F-G Hygro c1

Plasmid

#54463

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

hygromycin phosphotranferase (hptII) (pCAMBIA)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP13A2_Halo_N_allele

Plasmid

#178138

Purpose

Donor vector for endogenous tagging of human ATP13A2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ATP13A2 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LI A-C 35S c2

Plasmid

#54409

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

cauliflower mosaic virus 35S promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID8Z

Plasmid

#165053

Purpose

Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LI C-D 1-10GFP

Plasmid

#54461

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 1-10 domain

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP685-2

Plasmid

#99490

Purpose

TEV::eGFP::ER::6xHis::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::6Xhis::linker::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
dg264_pENTR_ChronosCel

Plasmid

#66102

Purpose

Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

Depositor

Dominique Glauser

Article

Schild et al Genetics. 2015 May 28. pii: genetics.115

Insert

Chronos

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CSF1R_Halo_C_allele

Plasmid

#178139

Purpose

Donor vector for endogenous tagging of human CSF1R at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (CSF1R Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LI E-F 35S term c1

Plasmid

#54442

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Terminator of Cauliflower mosaic virus CaMV

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI B-C mCherry

Plasmid

#54426

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mCherry + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI B-C GFP

Plasmid

#54422

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

sGFP + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LI D-E GFP + linker c3

Plasmid

#54410

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + GFP

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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