We narrowed to 8,982 results for: ef1;

1. lenti EF1a-FLAG-dCas9-VPR

   Plasmid

   #114195

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-mCherry-IRES-Dre

   Plasmid

   #55633

   Purpose

   Expresses Dre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Inserts

   mCherry
   Dre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

   Plasmid

   #202608

   Purpose

   Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-Kv
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1-Luc2-P2A-tdTomato

   Plasmid

   #72486

   Purpose

   Express Luc2 and tdTomato under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-U6-gRNA-EF1a-EGFP-CAAX

   Plasmid

   #167936

   Purpose

   gRNA entry vector

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   U6-gRNA backbone
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

   Plasmid

   #160141

   Purpose

   Cre- dependent expression of Sun1GFP for nuclei isolation

   Depositor

   Andreas Pfenning

   Article

   Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

   Insert

   Sun1-GFP (Sun1 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N-truncated: removed amino acids 1-207, retained …

   Promoter

   Ef1a

   Available since

   March 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid

    #72485

    Purpose

    Express Luc2 and copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pC0046-EF1a-PspCas13b-NES-HIV

    Plasmid

    #103862

    Purpose

    Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pHage2-EF1a-TERT-IRES-Puro

    Plasmid

    #182590

    Purpose

    expresses TERT in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-ASAP3Kv-WPRE

    Plasmid

    #132330

    Purpose

    expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3KV
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Mutation

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti EF1a GFP-mCherry-mPlin1

    Plasmid

    #189005

    Purpose

    Fluorescent reportor for lipophagy

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    GFP, mChery, Plin1 (Plin1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid

    #170236

    Purpose

    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S176E, S180E

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3-EF1A-MYC-IRES-luc

    Plasmid

    #129775

    Purpose

    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

    Depositor

    Amaia Lujambio

    Article

    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

    Insert

    human MYC (MYC Human)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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