We narrowed to 5,538 results for: pcas

1. pCAG-HA-Nrxn1beta AS4(-)

   Plasmid

   #59409

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(-) (Nrxn1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-mCherry2-mito

   Plasmid

   #164164

   Purpose

   Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mCherry2-mtActA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-SspB-mScarlet-I

   Plasmid

   #178521

   Purpose

   Expresses SspB-mScarlet-I in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   SspB-mScarlet-I
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG tubulin GFP

   Plasmid

   #66105

   Purpose

   expression of chicken tubulin GFP

   Depositor

   Martin Scaal, Felicitas Proels

   Article

   et al Development. 2015 Feb 15;142(4):665-71.

   Insert

   tubulin
   

   Use

   Chicken expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

5. NLS-SpCas9mSA-His

   Plasmid

   #182951

   Purpose

   Express SpCas9mSA in bacteria

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   NLS-SpCas9mSA-His
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAX-EGFP-CIP4

   Plasmid

   #178363

   Purpose

   Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

   Depositor

   Erik Dent

   Article

   Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

   Insert

   Cdc42 Interacting Protein 4 (TRIP10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-I-SceI-Trex2

   Plasmid

   #44024

   Depositor

   Jeremy Stark

   Article

   Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

   Insert

   I-SceI fused to mTREX2
   

   Use

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA24N-ligase-cTF

    Plasmid

    #87743

    Purpose

    IPTG-inducible expression of ligase-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and cTF (chimeric transcription factor base…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459V2.0-SpCas9-HF1

    Plasmid

    #108293

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-FuG-E (P440E)

    Plasmid

    #119978

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E (P440E) envelope coating virus particle with NeuRet.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci Methods. 2018 Oct 19;311:147-

    Insert

    Fusion Glycoprotein type E (P440E)
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAX APP AENATA

    Plasmid

    #30144

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YENPTY-->AENATA

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBW842_pCAG-pExpr-FP-DECODER

    Plasmid

    #87551

    Purpose

    Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP, EGFP, iRFP720, mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-MCS-EGFP

    Plasmid

    #164092

    Purpose

    expression of EGFP-tagged proteins under CAG promoter

    Depositor

    Maik Hintze

    Insert

    EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-cofilin-S3D

    Plasmid

    #78297

    Purpose

    Mammalian expression of mouse cofilin S3D fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3D

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-SRGAP3-EGFP

    Plasmid

    #168504

    Purpose

    Expression of full length SRGAP3 with a C-terminal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP3 (SRGAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge