We narrowed to 14 results for: pcas

1. The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

   Plasmid Kit

   #1000000210

   Purpose

   Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

   Depositor

   Vincent Martin

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

2. CRISPRecise Kit

   Plasmid Kit

   #1000000235

   Purpose

   CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.

   Depositor

   Ervin Welker

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

3. CRISPaint Gene Tagging Kit

   Plasmid Kit

   #1000000086

   Purpose

   Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

   Depositor

   Veit Hornung

   Available since

   Sept. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Diatom uLoop Assembly Kit

   Plasmid Kit

   #1000000238

   Purpose

   The collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.

   Depositor

   Christopher Dupont

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

5. The Expanded CRISPEY Kit

   Plasmid Kit

   #1000000257

   Purpose

   Use the Expanded CRISPEY Kit for CRISPR-based gene editing of S. cerevisiae.

   Depositor

   Jeffrey Lewis

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

6. CyanoGate Kit

   Plasmid Kit

   #1000000146

   Purpose

   The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

   Depositor

   Alistair McCormick

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

7. Ubiquiton Yeast Kit

   Plasmid Kit

   #1000000243

   Purpose

   The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.

   Depositor

   Helle Ulrich

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

8. MoClo Plant Parts III: Transformation & Genome Engineering Kit

   Plasmid Kit

   #1000000236

   Purpose

   The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

   Depositor

   Daniel Voytas

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

9. Cultivarium POSSUM Toolkit

   Plasmid Kit

   #1000000234

   Purpose

   The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.

   Depositor

   Nili Ostrov

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

10. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit

    #1000000030

    Purpose

    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

    Depositor

    Takashi Yamamoto

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

11. Rinehart & Söll Phosphoprotein Synthesis Kit

    Plasmid Kit

    #1000000020

    Purpose

    Allows Sep incorporation (directed by UAG) into a protein by an E. coli strain that harbors a Sep-accepting tRNA (tRNASep), its cognate Sep–tRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep).

    Depositor

    Dieter Söll, Jesse Rinehart

    Available since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Yeast Secrete and Detect

    Plasmid Kit

    #1000000166

    Purpose

    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.

    Depositor

    Sylvestre Marillonnet

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

13. Human Kinase Domain Constructs for Automated Bacterial Expression

    Plasmid Kit

    #1000000094

    Purpose

    68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

    Plasmid Kit

    #1000000171

    Purpose

    Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

    Depositor

    Johannes Stuttmann, Sylvestre Marillonnet

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only