We narrowed to 18,398 results for: Mut

1. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-AasgRNA3.8

   Plasmid

   #121958

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b single chimeric gRNA, short version
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-FRBx2

   Plasmid

   #193571

   Purpose

   Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   mCherry
   

   Use

   Tags

   FRB (T2098L)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOXA9-MSCV-full-GFP

   Plasmid

   #20977

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

   Insert

   HOXA9 (Hoxa9 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Full length cDNA with ~1.1kb 3'UTR with 2 pu…

   Promoter

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. PKCalpha.K/N.FLAG

   Plasmid

   #10806

   Depositor

   Alex Toker

   Article

   Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

   Insert

   PKC alpha (PRKCA Bovine)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Kinase dead mutant: K368N (Lys 368 to Asn)

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

6. YIplac211-Erg11-iGFPx3

   Plasmid

   #115422

   Purpose

   Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   Erg11-iGFPx3
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   Endogenous

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pReporter_2

   Plasmid

   #60563

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. BRAF - A12G in pENTR

   Plasmid

   #216606

   Purpose

   gateway cloning donor vector containing BRAF ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   BRAF (BRAF Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   A12G

   Promoter

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMD19T-slr0230-PL22-sgRNANT1-KmR

   Plasmid

   #73224

   Purpose

   Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   PL22-sgRNA NT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PL22

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-DEST-R3R4(R)

    Plasmid

    #141010

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-RNF126 C231A

    Plasmid

    #138648

    Purpose

    Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

    Depositor

    Jane McGlade

    Article

    Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

    Insert

    RNF126 (RNF126 Mouse)
    

    Use

    Tags

    HA-HA

    Expression

    Mammalian

    Mutation

    C231A

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FRB-LplA(W37V)

    Plasmid

    #35608

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37V)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-EPN-01(ΔL1)

    Plasmid

    #80049

    Purpose

    Expression of EPN-01 mutated in TSG101 binding site.

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(deltaPTAP)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP10AAAA

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR-P5P4

    Plasmid

    #141064

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flag-YOD1 C160S

    Plasmid

    #49785

    Purpose

    Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    Flag-tag

    Expression

    Mutation

    C160S

    Promoter

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pegRNA_LRRK2-3

    Plasmid

    #199272

    Purpose

    pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LRRK2 6055GtoA pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGPΩTp

    Plasmid

    #130660

    Purpose

    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.

    Depositor

    Miguel Valvano

    Article

    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep

    Insert

    Ω trimethoprim cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Addition of Ω sites next to trimethoprim cassette…

    Promoter

    dhfr promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry

    Plasmid

    #107246

    Purpose

    hM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.

    Depositor

    Bryan Roth

    Article

    Roth et al bioRxiv 251769

    Insert

    hM3DREADD
    

    Use

    AAV

    Tags

    ABIcs, FLAG, and mCherry

    Expression

    Mutation

    T to D and S to D mutations in V2-tail

    Promoter

    hSYN

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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