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pZS220

Plasmid

#177642

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K47A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Expression

Yeast

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEB2-TagRFP

Plasmid

#103997

Purpose

Plasmid encoding TagRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUG-UBA1

Plasmid

#104665

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-ciBAR1

Plasmid

#200440

Purpose

Expresses Flag-tagged human ciBAR1 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

Insert

ciBAR1

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-ciBAR2

Plasmid

#200441

Purpose

Expresses Flag-tagged human ciBAR2 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

Insert

ciBAR2

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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DVK_AF

Plasmid

#66068

Purpose

MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only
pXY1

Plasmid

#90487

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXY1-spc

Plasmid

#90488

Purpose

Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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perbB1Y1045F-EGFP

Plasmid

#40267

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB1 (EGFR Human)

Tags

EGFP

Expression

Mammalian

Mutation

Tyr1045>Phe

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pUG-UBC8

Plasmid

#104673

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC8 (UBC8 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+24)

Plasmid

#199163

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+24)

Tags

MGHHHHHHGGA

Mutation

E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgLmnb2-HepmCherry

Plasmid

#192830

Purpose

Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-HaloTag-DRS2

Plasmid

#177103

Purpose

Expresses HaloTag-Drs2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

DRS2 (DRS2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean ME

Plasmid

#103971

Purpose

Plasmid encoding mCerulean ME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean ME

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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CaYin1 NES

Plasmid

#33318

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1 NES

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCAGEN-SBP-DICER1

Plasmid

#50558

Purpose

purification of Dicer1 from mammalian cells

Depositor

Yukihide Tomari

Article

Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

Insert

Dicer1 (Dicer1 Mouse)

Tags

SBP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FRB-LplA(W37V)

Plasmid

#35608

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37V)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01

Plasmid

#80046

Purpose

expression of EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Tags

Myc

Expression

Mammalian

Mutation

M1I

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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