We narrowed to 15,989 results for: LEA

1. pDest17_Gcn4 ILVtoAS

   Plasmid

   #231859

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoAS
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   L123S, I128S, V130A, V135A, L137S, I142S

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest17_Gcn4 ILVtoED

   Plasmid

   #231857

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   L123D, I128E, V130E, V135E, L137D, I142D

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTYB1-T4gp47

   Plasmid

   #228212

   Purpose

   Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

   Depositor

   Stephen Benkovic

   Article

   Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

   Insert

   47 (47 Escherichia phage T4)
   

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-hfYFP-TEV-mScarlet-i

   Plasmid

   #191196

   Purpose

   Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & mScarlet-I fusion
   

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-LSSmGFP-TEV-mScarlet-i

   Plasmid

   #191199

   Purpose

   Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & mScarlet-I fusion
   

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. R152C USP7 TRAF

   Plasmid

   #227229

   Purpose

   Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   R152C; aa 62-205

   Promoter

   T7-promoter

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. G392A FL USP7

   Plasmid

   #227217

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   G392A

   Promoter

   T7-promoter

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. N418S FL USP7

   Plasmid

   #227215

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   N418S

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. H111Q USP7 TRAF

   Plasmid

   #227227

   Purpose

   Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   H111Q; aa 62-205

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. G392D FL USP7

    Plasmid

    #227216

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392D

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. K420E FL USP7

    Plasmid

    #227223

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    K420E

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. L373F FL USP7

    Plasmid

    #227220

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L373F

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Y331H FL USP7

    Plasmid

    #227218

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Y331H

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P273H FL USP7

    Plasmid

    #227221

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    P273H

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. M225I FL USP7

    Plasmid

    #227219

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    M225I

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. F118L USP7 TRAF

    Plasmid

    #227228

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    F118L; aa 62-205

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L469R FL USP7

    Plasmid

    #227225

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L469R

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. E345K FL USP7

    Plasmid

    #227224

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    E345K

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. V485G FL USP7

    Plasmid

    #227222

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    V485G

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAC3-C-GFP-NLS

    Plasmid

    #222604

    Purpose

    Expression of MAC3-tagged GFP-NLS (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
    

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Aug. 6, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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