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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Tags

myc3

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA Hygro

Plasmid

#167907

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Plasmid

#87644

Purpose

For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::SNAPf>myr::CLIPf>myr::Halo2

Expression

Insect

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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Dead-Laconic/pcDNA3.1(-)

Plasmid

#118627

Purpose

To express in mammalian cells a lactate FRET sensor negative control.

Depositor

Luis Felipe Barros

Article

Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

Insert

Dead-Laconic

Expression

Mammalian

Mutation

Change Histidine 151 to Aspartic Acid of Escheric…

Promoter

CMV Promoter

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-EAMP

Plasmid

#138265

Purpose

mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

iCas9 target-mCherry

Use

CRISPR

Expression

Mammalian

Promoter

EF1a-HTLV

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Bi-β-chain

Plasmid

#133731

Purpose

Expresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Inserts

b-Chain_gePSI
ZsGreen1

Expression

Mammalian

Mutation

Only one part of the gePSI-system (startcodon + a…

Promoter

pTRE3G-Bi

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM5-N-22

Plasmid

#54170

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM5

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-MCM7-N-22

Plasmid

#54174

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM7

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pK031.TRE-Cre (Supernova)

Plasmid

#69136

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

NLS-Cre

Expression

Mammalian

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pVL1392 TMEM97

Plasmid

#157748

Purpose

Baculovirus transfer vectors to create a virus expressing human TMEM97

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Tags

flag

Expression

Insect

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET12a-PAPI

Plasmid

#19961

Depositor

Jonathan Tait

Article

Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

Insert

Annexin V (ANXA5 Human)

Expression

Bacterial

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ArcLight-A242

Plasmid

#36857

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

semian CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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hSyn1-sypHy-RGECO

Plasmid

#84078

Purpose

Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

Depositor

Juan Burrone

Article

Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

Insert

sypHy-RGECO

Expression

Mammalian

Promoter

hSyn1

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

Plasmid

#176573

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Avitag, Hexahistidine tag, and mEGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMF-TetTight

Plasmid

#193288

Purpose

Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

rtTA-P2A-tTS

Use

Lentiviral

Expression

Mammalian

Promoter

EF‐1α

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBOB-synP-HTB

Plasmid

#30195

Depositor

Edward Callaway, Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

histone-GFP-2A-TVA receptor-2A-rabies glycoprotein

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 HaloTag-V5-TurboID

Plasmid

#226474

Purpose

Expresses fusion of HaloTag and TurboID in mammalian cells

Depositor

Hyun-Woo Rhee

Article

Kwak et al Cell Chem Biol. 2022 Dec 15;29(12):1739-

Insert

TurboID

Tags

HaloTag and V5

Expression

Mammalian

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-WIPI-1

Plasmid

#38272

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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