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pNR220

Plasmid

#79541

Purpose

Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

TP901
BxbI
A118

Tags

His tag and LAA degradation tag

Expression

Bacterial

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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HRE-dUnaG

Plasmid

#201711

Purpose

Hypoxia responsive promoter driving destablized UnaG fluorescent protein

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_MCP-p300_Hygro

Plasmid

#138459

Purpose

lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

p300 core

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFS555

Plasmid

#242855

Purpose

This plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locus

Depositor

Nick Rhind

Article

Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791

Inserts

TetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW4 promoter

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

Plasmid

#202689

Purpose

CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Inserts

CopGFP
mCherry

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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3n1 (572)

Plasmid

#70727

Purpose

BetI/PhlF/SrpR BCD7 3-node v41-1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
betI-ssrA
phlF-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pJAT32

Plasmid

#204297

Purpose

pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0013

Plasmid

#205582

Purpose

NpuDnaE(C)_HYG(53-341)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p285 TIR1_HA_mH11

Plasmid

#164855

Purpose

constitutive TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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AKARet-cyto

Plasmid

#96897

Purpose

FLIM sensor for PKA activation in cytosol

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for PKA Activity in Cytosol

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PRExpress

Plasmid

#122486

Purpose

A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Inserts

LacZ
poly-PRE

Expression

Insect

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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luxA+luxB/pRSETB Duet

Plasmid

#197581

Purpose

Expressing luxA and luxB under dual T7 promoter

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxB

Tags

T7 tag and 6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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DS-SPcas

Plasmid

#48645

Purpose

Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
tracrRNA precursor

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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EKARet-cyto

Plasmid

#96895

Purpose

FLIM sensor for ERK activation in cytosol

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for ERK Activity in Cytosol

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2186

Plasmid

#40386

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

WT aa1867-2186

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pDS1928

Plasmid

#167284

Purpose

Expression plasmid for Candida albicans containing a red-shifted luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Inserts

red shifted luciferase
SAT1

Promoter

ACT1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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N114 nLV EF-1a-Gal4-VP16-PGK-Puro

Plasmid

#44014

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Gal4/DBD (1-147)

Use

Lentiviral

Tags

VP16/Activation/Domain

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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