Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,533 results for: Gaa

Showing: 1121 - 1140 of 4533 results

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pAvA141

Plasmid

#248146

Purpose

mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations

Depositor

Emil Jensen

Article

van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s

Insert

luxR (gen2 mutations)

Use

Integration vector

Tags

Gal4 activation domain and nuclear localization s…

Expression

Yeast

Mutation

Gal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135V

Promoter

pPGK1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-d5-HT1BR

Plasmid

#221821

Purpose

Plasmid to express gRNA1 (gaaaatttgatttcaactga) for editing at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1BR gRNA1 (5-HT1B Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-5-HT2AR-isoformD

Plasmid

#221824

Purpose

Plasmid to express gRNA2 (ctgaagacataattacgtgg) for editing at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoform D gRNA2 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-5-HT2AR-isoformBFH

Plasmid

#221826

Purpose

Plasmid to express gRNA2 (ggaaaagccgctaattacag) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoforms BFH gRNA2 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_NRF2-exon5-1

Plasmid

#177781

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

Depositor

Michael Ristow

Article

Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

Insert

NRF2 (NFE2L2 Human)

Use

CRISPR

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_NRF2-exon5-2

Plasmid

#177782

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

Depositor

Michael Ristow

Article

Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

Insert

NRF2 (NFE2L2 Human)

Use

CRISPR

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ECS-dSpyCatcher003

Plasmid

#234963

Purpose

Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

None

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-4mer-30kb-DSF

Plasmid

#227485

Purpose

4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 30kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-3mer-30kb-DSF

Plasmid

#227486

Purpose

3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 30kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-35kb-DSF-1-6

Plasmid

#227487

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 35kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-44kb-DSF

Plasmid

#227494

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 44kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-48kb-DSF

Plasmid

#227495

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 48kb Downstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-3.3kb-USF

Plasmid

#227475

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 3.3kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-2.4kb-USF

Plasmid

#227477

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 2.4kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pk335-CARGO-6mer-2.1kb-USP

Plasmid

#227451

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 2.1kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-700bp-USP

Plasmid

#227452

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 700bp Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-5mer-PrPro

Plasmid

#227454

Purpose

5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to Prdm8 promoter Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-4mer-PrPro

Plasmid

#227455

Purpose

4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to Prdm8 promoter Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pk335-CARGO-6mer-55kb-USF

Plasmid

#227458

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 55kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pk335-CARGO-6mer-36kb-USF

Plasmid

#227461

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 36kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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