We narrowed to 166,097 results for: Gene

1. sgRNA targeting human SLC12A5 gene stop codon

   Plasmid

   #158577

   Purpose

   For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   sgRNA targeting human SLC12A5 gene stop codon
   

   Use

   CRISPR

   Tags

   Cas9 and GFP

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSE163: pFuGW-SSX1p-GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS

   Plasmid

   #240457

   Purpose

   A synthetic transcription factor (synTF) that contains a synthetic general 3CLpro cleavage site (CS)

   Depositor

   Lior Nissim

   Article

   Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

   Inserts

   SSX1p
   GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS
   

   Use

   Lentiviral

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE5: pFuGW-SSX1p- GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS

   Plasmid

   #240460

   Purpose

   a synthetic transcription factor (synTF) that contains 4 tandem synthetic general 3CLpro cleavage site (CS).

   Depositor

   Lior Nissim

   Article

   Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

   Inserts

   SSX1p
   GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS
   

   Use

   Lentiviral

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

   Plasmid

   #60815

   Purpose

   dCas9 with single cysteine residue (M1C) for site-specific labelling

   Depositor

   Jennifer Doudna

   Article

   O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

   Insert

   dCas9 M1C D10A C80S H840A C574S
   

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   M1C D10A C80S H840A C574S

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest17_Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCD (1-136)

   Plasmid

   #235001

   Purpose

   Bacterial expression of N-terminally 6His tagged Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCD

   Depositor

   Tanja Mittag

   Insert

   Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein (LOC129490717 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210739

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 3
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210737

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 1
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210738

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 2
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Promoter

   H1 and eCMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210740

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 4
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Promoter

   H1 and eCMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pttq18

    Plasmid

    #69122

    Purpose

    regulated expression of genes in Escherichia coli

    Depositor

    Michael Stark

    Article

    Stark et al Gene. 1987;51(2-3):255-67.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVU/myc

    Plasmid

    #24179

    Depositor

    Lars Ittner

    Insert

    Chloramphenicol resistance gene (CmR) - ccdB gene
    

    Use

    Lentiviral; Gateway

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pDGM3B

    Plasmid

    #110809

    Purpose

    contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

    Depositor

    David Russell

    Article

    Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

    Insert

    AAV3B cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW32

    Plasmid

    #110225

    Purpose

    integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW33

    Plasmid

    #110226

    Purpose

    integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAW34

    Plasmid

    #110227

    Purpose

    integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJQ200mp18

    Plasmid

    #78500

    Purpose

    Suicide vector for gene replacement

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector for gene replacement

    Promoter

    none

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAW41

    Plasmid

    #110228

    Purpose

    integration of loxP site a distance upstream of gene start site

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Insert

    ura4+ (ura4 Fission Yeast)
    

    Use

    Cre/Lox

    Mutation

    See depositor comments below.

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW5

    Plasmid

    #110221

    Purpose

    Cre-expression plasmid

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW8

    Plasmid

    #110222

    Purpose

    Cre-expression plasmid for cassette exchange

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJQ200uc1

    Plasmid

    #78502

    Purpose

    Suicide vector for gene replacement, modified MCS to maximise utility of insert restriction sites.

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector for gene replacement

    Tags

    none

    Promoter

    none

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only