Addgene

We narrowed to 1,226 results for: SUMO

Showing: 1121 - 1140 of 1226 results

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pGP-AAV-syn-iGABASnFR2(no bind)-WPRE

Plasmid

#218876

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2(no bind)

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

Synapsin

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170813

Purpose

Lentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170815

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP Kindlin2

Plasmid

#105305

Purpose

To study effect of Kindlin 2 on Cell/ECM adhesion

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMT899

Plasmid

#228288

Purpose

Constitutive mutant rPhlTA expression with strong promoter

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

rphlTA mutant

Use

Synthetic Biology

Tags

Three copies of VP16 (VP48)-Nuclear localization …

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K

Promoter

KpGAPDH promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-flex-iGABASnFR2-WPRE

Plasmid

#218877

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

Synapsin

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA Myc DBC1

Plasmid

#35096

Depositor

Osamu Hiraike

Article

Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

Insert

deleted in breast cancer 1 (CCAR2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE

Plasmid

#218879

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Has Service

AAV1 and AAV5

Insert

iGABASnFR2(no bind)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

Synapsin

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PdTK-CAG-mCh-[uBglII]

Plasmid

#107280

Purpose

puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )

Use

Tags

Expression

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRL838

Plasmid

#70680

Purpose

This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

Depositor

Peter Wolk

Article

Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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LRRK2-K1906M

Plasmid

#35946

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

LRRK2 (LRRK2 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-SENP2 C548A

Plasmid

#126592

Purpose

Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C548A

Promoter

CMV

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 WT

Plasmid

#126591

Purpose

Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant to GAGGAGAUAUUCAGACAUU

Promoter

CMV

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP AH2 tensin

Plasmid

#50514

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.

Insert

Tensin (TNS1 Chicken)

Use

Tags

GFP

Expression

Mammalian

Mutation

AA659--761, including actin homology 2 domain of …

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-TDP-43 (2KR)

Plasmid

#235494

Purpose

Expression of human TDP-43-EGFP with K136R and K408R mutations

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K136R, K408R

Promoter

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-TDP-43

Plasmid

#235487

Purpose

Expression of human TDP-43-EGFP

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-TDP-43 (K136R)

Plasmid

#235492

Purpose

Expression of human TDP-43-EGFP with K136R mutation

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K136R

Promoter

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-TDP-43-HA

Plasmid

#235496

Purpose

Expression of human TDP-43-HA fusion protein

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pYI2294

Plasmid

#228352

Purpose

Methanol-inducible ALX-0081 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Mutation

Promoter

KpAOX1 promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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