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  1. Plasmids 101: Protein tags

    Type
    Blog Post
    ...site. In fact, SUMO cannot be used in eukaryotes because there is already too much SUMO protease around... its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can... solubility; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus... your friend rather your enemy. Two common tags (SUMO and FLAG) are cleaved by specific proteases without...with purified protein since the enzyme cleaves the SUMO tag in the same manner as it would have in the context...

  4. Bacterial Expression Systems

    Type
    Collection
    ...repressible) His, Strep, mOCR, MBP, with and without SUMO or TEV cleavage sites to remove tags Scott Gradia...Flag, His, Strep, mOCR, MBP, GFP, with and without SUMO or TEV cleavage sites to remove tags. Scott Gradia...combinations of N and C-terminal Strep, His, MBP, TRX, GST, SUMO, MISTIC, and LSL tags as well as tags for protein...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...the same animal. Flipbow additionally incorporates SUMO tags in the FP sequence for separation from Cre-...S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, solubilization and purification...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...disease Thomas Tuschl 107292 1a. pETM11-SUMO-SNCA-GFP SNCA His, SUMO T7 Parkinson's Dmytro Yushchenko 107293...pHBS834 H14-SUMO-TDP43 WT-TEV-mCherry TARDBP T5 ALS Rajat Rohatgi 133321 pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry... TARDBP T5 ALS Rajat Rohatgi 133322 pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry TARDBP T5 ALS Rajat Rohatgi...HNRNPA1 His, mCherry T7 ALS Samie Jaffrey 205054 SUMO-Twinkle 43-372 TWNK His T7 Spinocerebellar ataxia...ataxia, infantile onset Smita Patel 205055 SUMO-Twinkle 360-684 TWNK His T7 Spinocerebellar ataxia, infantile...infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His Spinocerebellar ataxia, infantile onset Smita...Parkinson's Michael J Fox Foundation MJFF 40386 pBACgus_Sumostar/LRRK2 1867-2186 LRRK2 His, TEV polH Parkinson's...
Showing: 1 - 6 of 6 results