We narrowed to 5,538 results for: pcas

1. ER50-SpCas9-ER50

   Plasmid

   #85448

   Purpose

   Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   ER50-SpCas9-ER50
   

   Use

   AAV

   Tags

   destabilized domain of estrogen receptor ligand b…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAX-mScarlet-FBP17

   Plasmid

   #178366

   Purpose

   Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

   Depositor

   Erik Dent

   Article

   Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

   Insert

   Formin Binding Protein 17 (FNBP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   400 Glutamic Acid changed to Aspartic acid (403 A…

   Promoter

   Chicken Beta Actin

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-SRGAP1-Myc

   Plasmid

   #168503

   Purpose

   Expression of full length SRGAP1 with a C-terminal Myc fusion

   Depositor

   Franck Polleux

   Article

   Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

   Insert

   SRGAP1 (SRGAP1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   aa 1-501

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mScarlet-Giantin

   Plasmid

   #196872

   Purpose

   Expresion of Giantin-fused to mScarlet. Used as Golgi marker

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mScarlet-Giantin (GOLGB1 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-FLAG-CaMKIIG

   Plasmid

   #186828

   Purpose

   Expresses FLAG-CaMKIIG in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2g (Camk2g Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAX-EGFP-FBP17

   Plasmid

   #178365

   Purpose

   Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

   Depositor

   Erik Dent

   Article

   Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

   Insert

   Formin Binding Protein 17 (FNBP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta Actin

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tubp-Gal4 in pCaSpeR4

   Plasmid

   #17747

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Tubulin 1alpha promoter and hsp70 PolyA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCAGGS-6His-mISG15

   Plasmid

   #12445

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

9. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

   Plasmid

   #86612

   Purpose

   Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Myc-Oct3/4-IP

    Plasmid

    #13460

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Myc (6x)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNS20-SpCas9-SNAP

    Plasmid

    #113717

    Purpose

    Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-VPS15-TSF

    Plasmid

    #203563

    Purpose

    Mammalian expression of VPS15 with TwinStrep-Flag tag

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    VPS15 (PIK3R4 Human)
    

    Use

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAIP-hXRCC1pd-eGFP

    Plasmid

    #206033

    Purpose

    Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    CAG

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-mCherry-ATG14

    Plasmid

    #203567

    Purpose

    Mammalian expression of mCherry tagged ATG14

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAT-YFP-TetR

    Plasmid

    #59018

    Purpose

    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

    Depositor

    David Sibley

    Article

    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

    Insert

    YFP-TetR
    

    Use

    Toxoplasma expression

    Tags

    Expression

    Mutation

    Promoter

    alpha-tubulin

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Flp-DOG

    Plasmid

    #80084

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    Flp-DOG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG_Xph18-eGFP-CCR5TC

    Plasmid

    #135529

    Purpose

    Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph18
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAS9-BFP empty

    Plasmid

    #80976

    Purpose

    Cas9, TagBFP, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-GST-ATG14

    Plasmid

    #203566

    Purpose

    Mammalian expression of GST tagged ATG14

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT224_(pCA-tTA2)

    Plasmid

    #36430

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tetracycline transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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