We narrowed to 5,840 results for: pcas

1. pCAP03-sp44/p21

   Plasmid

   #120232

   Purpose

   Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
   

   Use

   Synthetic Biology

   Mutation

   aac(3)IV (apramycin resistance gene)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-I-SceI-Trex2

   Plasmid

   #44024

   Depositor

   Jeremy Stark

   Article

   Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

   Insert

   I-SceI fused to mTREX2
   

   Tags

   NLS-HA

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCARGO-RMCE-Xist

   Plasmid

   #233268

   Purpose

   CARGO plasmid containing ~22kb dox-inducible Xist transgene

   Depositor

   Mauro Calabrese

   Article

   Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

   Insert

   22kb Xist gene, including introns
   

   Expression

   Mammalian

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG2-EGFP-C-IFT122

   Plasmid

   #218737

   Purpose

   Expresses C-terminally EGFP-tagged IFT122 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT122 (IFT122 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_Xph15-SNAPf-CCR5TC

   Plasmid

   #135536

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Tags

   SNAPf

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HiBiT-CD63

   Plasmid

   #162591

   Purpose

   N-terminally HiBiT-tagged human CD63 under CAG promoter

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

   Insert

   human CD63 (CD63 human, Synthetic, Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 35S::GeNL/pCAMBIA1301

   Plasmid

   #190070

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Green-enhanced Nano-lantern
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Mutation

   codon optimized for human

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-EGFP-SOS1

    Plasmid

    #178860

    Purpose

    Mammalian expression of SOS1 fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    SOS1 (SOS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-PPP1CB-miRFP703

    Plasmid

    #178524

    Purpose

    Expresses PP1c-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    PP1c-miRFP703 (PPP1CB Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCambia2300 - ProRUBY:RUBY-Citrine

    Plasmid

    #128796

    Purpose

    Fluorescent reporter for subcellular localisation and expression analysis of RUBY

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

    Insert

    RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)
    

    Tags

    Citrine

    Expression

    Plant

    Promoter

    ProRUBY

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-cofilin-S3A

    Plasmid

    #78296

    Purpose

    Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3A

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMUT2-pCadBA-mCherry

    Plasmid

    #192865

    Purpose

    pMUT2 based vector for sensing pH in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    pH sensitive promoter
    

    Expression

    Bacterial

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-NRCH-SsAPOBEC3B

    Plasmid

    #198550

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459V2.0-SpCas9-HF1

    Plasmid

    #108293

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-b3STOPb3-ZsGreen

    Plasmid

    #51266

    Purpose

    B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    b3STOPb3-ZsGreen
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. spCas9-NLS-MAV

    Plasmid

    #124212

    Purpose

    SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

    Depositor

    Uri David Akavia

    Article

    Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    His6, MBP, TEV Cleavage site, and two copies of S…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-memRFP-3xControlgRNA

    Plasmid

    #224567

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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