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pLY77

Plasmid

#130964

Purpose

Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY124

Plasmid

#130946

Purpose

Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-G6-2MS2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pbad

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY112

Plasmid

#130944

Purpose

Engineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY107

Plasmid

#130943

Purpose

Engineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY105

Plasmid

#130939

Purpose

Engineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA3

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY106

Plasmid

#130941

Purpose

Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC2

Plasmid

#131172

Purpose

Cloning vector containing A-C-B casette

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Type

Empty backbone

Expression

Mammalian

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1

Plasmid

#131171

Purpose

Cloning vector containing A-B-C casette

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Type

Empty backbone

Expression

Mammalian

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag

Plasmid

#129255

Purpose

Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST

Plasmid

#129264

Purpose

Express soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag-ST

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Plasmid

#129263

Purpose

Express soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Mac_Q139D_D150N_E258V-HaloTag

Plasmid

#129259

Purpose

Express Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag

Plasmid

#129258

Purpose

Express QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV689

Plasmid

#119016

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0023

Plasmid

#117538

Purpose

Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

sgRNA (Sp Cas9) - extended stem with U626 terminator

Expression

Plant

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0087

Plasmid

#117564

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtUUAT1 {AT5G04160} in A. thaliana

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sa_gRNA_UUAT1 {AT5G04160} A. thaliana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0114

Plasmid

#117553

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana

Expression

Plant

Promoter

At U6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0019

Plasmid

#117529

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.1 (no stop codon)

Use

CRISPR

Mutation

K848A, K1003A, R1060A

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0018

Plasmid

#117528

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.1

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.1 (with stop codon)

Use

CRISPR

Mutation

K848A, K1003A, R1060A

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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