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S6_BstBI_SV40_SwaI

Plasmid

#128470

Purpose

SV40 promoter

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

SV40 promoter

Expression

Bacterial

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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S5_EcoRV_Ef1a_AscI

Plasmid

#128469

Purpose

Ef1a promoter

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Ef1a promoter

Expression

Bacterial

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S12_EcoRV_PGK_AscI

Plasmid

#128476

Purpose

PGK promoter

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

PGK promoter

Expression

Bacterial

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_WTTS

Plasmid

#91241

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

W22H

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYCDuet_F31YG121VDHFR_WTTS

Plasmid

#91229

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121V

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_WTTS

Plasmid

#91233

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Publication

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NG

Plasmid

#90420

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NG

Depositor

Peter Kroth

Publication

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pM9_fcpA_NN

Plasmid

#90419

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NN

Depositor

Peter Kroth

Publication

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pM9_fcpA_NI

Plasmid

#90418

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NI

Depositor

Peter Kroth

Publication

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pM9_NR_NI

Plasmid

#90422

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NI

Depositor

Peter Kroth

Publication

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Expression

Mammalian

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVV647

Plasmid

#87630

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bxb1 attP

Use

CRISPR

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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jk57

Plasmid

#87010

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Publication

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

Nlg1AB
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode1

Plasmid

#83678

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Publication

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode1

Use

Retroviral

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGAPTrap-LacZ-IRESMuro

Plasmid

#82507

Purpose

Vector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Publication

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

LacZ-IRESMuro

Use

Synthetic Biology

Expression

Mammalian

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE3

Plasmid

#79442

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE64

Plasmid

#79446

Purpose

recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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