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pSKB-

Plasmid

#62540

Purpose

Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Bacterial

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

Plasmid

#92402

Purpose

Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Kalyviotis et al (2020) Behavioral and Neural Genetics of

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and No tag, cytosolic expression

Promoter

bactin2

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Chrm3_Csx30_Cre

Plasmid

#197603

Purpose

Cre reporter for mammalian cells with Chrm3 membrane anchor

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Chrm3_Csx30_Cre

Expression

Mammalian

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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DS-NMcas

Plasmid

#48646

Purpose

Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
NM tracrRNA

Use

CRISPR

Promoter

NM tracrRNA promoter and proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pAB168

Plasmid

#101671

Purpose

Opto-T7RNAP*(563-F1), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDB007(+)

Plasmid

#99147

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)

Expression

Bacterial

Promoter

PT7 and PproK

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-338

Plasmid

#68795

Purpose

Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2

Tags

IRES, NLS, flag, and nls

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDB038

Plasmid

#99210

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*), luxAB; PproK pylT

Expression

Bacterial

Mutation

amber codons at position 29 gIII

Promoter

Ppsp and PproK

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag- psa A Left -G1397_C ABE

Plasmid

#189646

Purpose

psa A Left -G1397_C ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Article

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pTORGm43GWR

Plasmid

#112905

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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p273 EF1a_mCherry2_hH11

Plasmid

#164852

Purpose

mCherry2-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

mCherry2

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pUG-amdSYM-mak10TR

Plasmid

#75433

Purpose

Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

Depositor

John McCusker

Inserts

MAK10TR
ApR
amdSYM

Expression

Yeast

Mutation

N-terminal 740 bp of MAK10 ORF

Promoter

Native promoter and PTEF

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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N-term Hec1 Plk1 FRET sensor c-jun substrate

Plasmid

#45204

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted Plk1 FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAXY0007

Plasmid

#205576

Purpose

NpuDnaE(C)-Glucos(232-500)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-Glucosyltransferase

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWC

Plasmid

#112903

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWF

Plasmid

#112904

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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