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pCAGEN-DEST (JDW 471)

Plasmid

#242573

Purpose

Gateway destination vector with a CAGGS promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway subcloning

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Nsp3(1-111)

Plasmid

#242947

Purpose

GFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)

Depositor

Benjamin Schuster

Article

Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

Insert

GFP-Nsp3(1-111) (ORF1ab SARS-CoV-2)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β6β1-EGFP

Plasmid

#242829

Purpose

Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)

Expression

Mammalian

Mutation

Integrin β6 chimera containing the cytoplasmic ta…

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Sema7A

Plasmid

#190644

Purpose

Expresses Mouse Sema7A with HA Tag

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pYO979

Plasmid

#235756

Purpose

Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Expression

Yeast

Mutation

FRK430-432DDD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pYO359

Plasmid

#235750

Purpose

Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S2A, aa 3-887 and T134A, I851R

Promoter

GAL-TDH3

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1124

Plasmid

#235725

Purpose

Expression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

UVRAG(1-464) fused to ATG14L BATS domain (413-492)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJB180

Plasmid

#235737

Purpose

Expression of mCherry- human RAB1A Q70L in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB1A (RAB1A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Q70L

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1006

Plasmid

#235724

Purpose

Expression of FL human Beclin 1 in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Insert

Beclin 1 (BECN1 Human)

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1296

Plasmid

#235710

Purpose

Expression of mCherry-human RAB5A Q79L in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5 (RAB5A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Q79L, aa 2-215

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-cCGNL1-Myc

Plasmid

#236513

Purpose

For expression of Paracingulin (dog) in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al Mol Membr Biol. 2008 Jan;25(1):1-13. doi

Insert

CGNL1 (CGNL1 Canis lupus familiaris (dog))

Tags

Myc and YFP

Expression

Mammalian

Mutation

WT

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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AAV PHP.eB-CAG-Venus

Plasmid

#233695

Purpose

AAV expression of Venus from CAG promoter

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Venus

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSMPPv2-Tau-RING

Plasmid

#233688

Purpose

Expression of Tau-RING protein in mammalian cells

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Tau protein fused to TRIM21 RING domain

Use

Lentiviral

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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HA-cNM2A-Flag

Plasmid

#236532

Purpose

For expression of NM2A (dog) - with HA and FLAG tags in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH9

Tags

FLAG and HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1262

Plasmid

#235727

Purpose

Protein expression of His6-RAB1A Q70L 1-203 in bacteria

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB1A (RAB1A Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Q70L, aa 1-203

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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PM-frNFAST

Plasmid

#233602

Purpose

Expression of frNFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

frNFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pB-DEST (JDW 1205)

Plasmid

#229821

Purpose

A PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Neo-DEST (JDW 940)

Plasmid

#229825

Purpose

A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-phiC31o (JDW 453)

Plasmid

#229842

Purpose

A CMV-driven Phi31 recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

phiC31 codon optimized, PhiC31o

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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GvpA-CaM

Plasmid

#223555

Purpose

Full length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.

Depositor

Jean Gariepy

Article

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

pET300-GvpACaM

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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