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pTCW009

Plasmid

#83523

Purpose

Aromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pARO9-mfalpha1

Expression

Yeast

Promoter

pARO9

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGS-3

Plasmid

#85094

Purpose

Host vector to fuse a gene at the N-terminus of PCNA3

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pC3

Plasmid

#85087

Purpose

Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

PCNA3 fused to P450cam

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTCW005

Plasmid

#83524

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-malpha1

Expression

Yeast

Promoter

pFUS1

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBW100ParsR

Plasmid

#78635

Purpose

pSB3K3 carrying J101-rbs32-arsR-t-ParsR

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J101-rbs32-arsR-t-ParsR

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBW205J105-gfp

Plasmid

#78644

Purpose

pSB3K3 carrying J105-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J105-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW203J116-gfp

Plasmid

#78642

Purpose

pSB3K3 carrying J116-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J116-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW202J114-gfp

Plasmid

#78641

Purpose

pSB3K3 carrying J114-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J114-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)

Plasmid

#70733

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLacI-BCD7-Citrine P42U18C68T11 v40-1 (536)

Plasmid

#70725

Purpose

Citrine reporter for transfer fxns

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-lambdaCI-ssrA P65U18C47T14 v40-2 (495)

Plasmid

#70735

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

lambdaCI-ssrA

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496)

Plasmid

#70737

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

lacI-ssrA

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)

Plasmid

#70739

Purpose

plasmid for building 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-6

Plasmid

#72738

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-124 + miR-128 + miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-124 + miR-128 + miR-132]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-19

Plasmid

#72751

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)

Plasmid

#70744

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean-ssrA

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-20

Plasmid

#72752

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-betI-ssrA P65U18C64T14 v50-2 (567)

Plasmid

#70734

Purpose

plasmid for building 3n1, 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

betI-ssrA

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-BCD7-qacR-ssrA P69U18C63T16 v50-2 (568)

Plasmid

#70732

Purpose

plasmid for building 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-BCD7-phlF-ssrA P69U18C51T15 v40-3 (570)

Plasmid

#70731

Purpose

plasmid for building 3n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge