Addgene

We narrowed to 7,585 results for: Lif;

Showing: 1141 - 1160 of 7585 results

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pcDNA4/TO-2xStrep-UL32

Plasmid

#162629

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2080

Plasmid

#155160

Purpose

Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_L266A

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2081

Plasmid

#155161

Purpose

Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF(Y261A_L265A_L266A)

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1305

Plasmid

#155143

Purpose

Expresses MlaFB, His tag on MlaF N-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(6xHis-TEV-MlaF)-MlaB

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1306

Plasmid

#155144

Purpose

Expresses MlaFB, His tag on MlaF C-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaF-6xHis)-MlaB

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1307

Plasmid

#155145

Purpose

Expresses MlaFB, His tag on MlaB N-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1308

Plasmid

#155146

Purpose

Expresses MlaFB, His tag on MlaB C-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(MlaB-6xHis)

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1833

Plasmid

#155149

Purpose

Expresses MlaF(1-250)-GCN4

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-250)-GCN4_Dimerization_Domain

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1957

Plasmid

#155151

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

added Streptavidin tag on MlaF N-terminus

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2074

Plasmid

#155155

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaB_T52E

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2076

Plasmid

#155156

Purpose

Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_K47A

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2077

Plasmid

#155157

Purpose

Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_E170Q

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2078

Plasmid

#155158

Purpose

Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_Y261A

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2079

Plasmid

#155159

Purpose

Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_L265A

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1244

Plasmid

#155138

Purpose

Expresses MlaFED operon in E. coli

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.a

Plasmid

#107523

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.a

Use

Tags

Expression

Mutation

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.b

Plasmid

#107524

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.b

Use

Tags

Expression

Mutation

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDC24

Plasmid

#131368

Purpose

expression of MEG-3Cterm (aa545-862) in C. elegans

Depositor

Geraldine Seydoux

Article

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa545-862) (meg-3 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDC21

Plasmid

#131367

Purpose

expression of MEG-3IDR (aa1-544) in C. elegans

Depositor

Geraldine Seydoux

Article

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa1-544) (meg-3 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A.pcDNA3-k

Plasmid

#126627

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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