We narrowed to 3,698 results for: Xpa

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pMK453 (BSD-P2A-dTAG-mCherry)

Plasmid

#214383

Purpose

dTAG-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Publication

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BSD-P2A-dTAG-mCherry

Use

CRISPR

Expression

Mammalian

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197024

Purpose

Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Publication

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized OsCas12f1

Expression

Mammalian

Promoter

CBh, CMV, hU6

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-CA TAG126

Plasmid

#105836

Purpose

Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

Depositor

Ryan Mehl

Publication

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 126

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-CA TAG93

Plasmid

#105667

Purpose

expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag

Depositor

Ryan Mehl

Publication

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 93

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMC8b-XI3up

Plasmid

#176170

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-3

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI3dw

Plasmid

#176171

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-3

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(84Q)-S776A

Plasmid

#21758

Depositor

Huda Zoghbi

Publication

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 84 Q(Glutamines) and…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pJW1821

Plasmid

#163092

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet^SEC (Lox511I)^::3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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RCASBP(A)-3xFlag-mHoxd13wt

Plasmid

#145288

Purpose

Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

Hoxd13(wt) (Hoxd13 Mouse)

Use

Viral avian expression

Tags

3xFlag

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-YFP-NLS

Plasmid

#145274

Purpose

Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCS and YFP

Expression

Mammalian

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1850

Plasmid

#154328

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145269

Purpose

Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TBP(38Q)-IDR (TBP Human)

Expression

Mammalian

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL-CP

Plasmid

#65715

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST

Use

Luciferase

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-2-G4BDMD-QFAD

Plasmid

#46091

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFAD

Expression

Insect

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pL59-Nat

Plasmid

#140465

Purpose

CRISPR/Cas9 engineering with nourseothricin selection

Depositor

Gianni Liti

Publication

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Yeast

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTXTL-T7max-LuxA

Plasmid

#188305

Purpose

Expression of LuxA via a T7 promoter in TXTL

Depositor

Kate Adamala

Publication

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luxA

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Plasmid

#165030

Purpose

Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Publication

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

T7

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ281 (MbCas12a)

Plasmid

#138113

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

MbCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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