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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 159,596 results for: addgene

Showing: 1141 - 1160 of 159596 results

  1. WNK3 (WNK3B-c016)

    Plasmid
    #110252
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK3 (WNK3 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110252-plasmid-map-sequence-id-212337

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  2. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  3. WNK1 (WNK1A-c011)

    Plasmid
    #98232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK1A (WNK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98232-plasmid-map-sequence-id-193412

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  4. pZBTB12.1.0-gDNA

    Plasmid
    #113769
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB12
    Depositor
    Kevin White
    Insert
    ZBTB12 (ZBTB12 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113769-plasmid-map-sequence-id-230555

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  5. pPyCAG-Nanog(L122A)-IP

    Plasmid
    #62992
    Purpose
    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog L122A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Leucine 122 (123 AA in Mouse Protein) to …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62992-plasmid-map-sequence-id-104441

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  6. pZBTB17.1.0-gDNA

    Plasmid
    #112483
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB17
    Depositor
    Kevin White
    Insert
    ZBTB17 (ZBTB17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112483-plasmid-map-sequence-id-230520

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  7. pSPDEF.1.0-gDNA

    Plasmid
    #113796
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SPDEF
    Depositor
    Kevin White
    Insert
    SPDEF (SPDEF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113796-plasmid-map-sequence-id-230613

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  8. pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)

    Plasmid
    #44087
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44087-plasmid-map-sequence-id-61673

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  9. pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)

    Plasmid
    #44078
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44078-plasmid-map-sequence-id-61691

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  10. pMLX.1.0-gDNA

    Plasmid
    #112466
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MLX
    Depositor
    Kevin White
    Insert
    MLX (MLX Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112466-plasmid-map-sequence-id-230496

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  11. pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)

    Plasmid
    #44088
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44088-plasmid-map-sequence-id-61682

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  12. pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

    Plasmid
    #44075
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44075-plasmid-map-sequence-id-61677

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  13. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  14. MLH1

    Plasmid
    #28120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-347.
    Promoter
    Available since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP37 (USP37 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)

    Plasmid
    #44066
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44066-plasmid-map-sequence-id-61687

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  18. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

    Plasmid
    #44069
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44069-plasmid-map-sequence-id-61666

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  19. pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)

    Plasmid
    #44064
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44064-plasmid-map-sequence-id-61680

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  20. OTU04

    Plasmid
    #28157
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OTU domain containing 7A (OTUD7A Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    UBA-like domain only
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1141 - 1160 of 159596 results