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pMBD-Gate4

Plasmid

#25960

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

GAL4 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMBD-Gate5

Plasmid

#25961

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

GAL4 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMBD-Gate6

Plasmid

#25962

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

GAL4 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pBS WSB2

Plasmid

#19190

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

WSB2 (WSB2 Chicken)

Expression

Bacterial

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pAJF1139

Plasmid

#252908

Purpose

For constitutive expression of GFP-NBALFA. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

GFP-NBALFA fusion

Tags

GFP

Expression

Bacterial

Promoter

PftsZ

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAJF1141

Plasmid

#252909

Purpose

For constitutive expression of GFP-NBALFA. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

GFP-NBALFA fusion

Tags

GFP

Expression

Bacterial

Promoter

PgroL

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAJF1120

Plasmid

#252903

Purpose

Tet-inducible GFP-NBALFA fusion plasmid. Contains oriM and kanamycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

GFP-NBALFA fusion

Tags

GFP

Expression

Bacterial

Promoter

Tet-inducible promoter

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Sec7-4V5-APEX2-URA

Plasmid

#207060

Purpose

Expressing Sec7-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Sec7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pSEC7

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Snf7-4V5-APEX2-URA

Plasmid

#207058

Purpose

Expressing Snf7-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pSNF7

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pDonorTp-fugfp

Plasmid

#211790

Purpose

pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

fuGFP

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pTGL3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pPEX3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHFdist_rm

Plasmid

#162318

Purpose

P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

IHF distal site is removed

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCB40

Plasmid

#205136

Purpose

Integrating expression of SnxA-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna2

Plasmid

#192796

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna2

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna6

Plasmid

#192797

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna6

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna6

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pProbe_CUSPER

Plasmid

#185953

Purpose

broad host plasmid that can be used to construct reproductive success bioreporter

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al ISME J. 2023 Sep;17(9):1445-1454. doi: 1

Insert

Unspecified

Expression

Bacterial

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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MiniSOG-SpAGS-GFP

Plasmid

#185581

Purpose

for CALI

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

miniSOG-SpAGS

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Cas9

Plasmid

#186723

Purpose

Transient expression of Cas9 in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Cas9

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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