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pTH1-SaSrtA-KQ

Plasmid

#64982

Purpose

Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108Q

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAD-g6

Plasmid

#61497

Purpose

For expression of C. briggsae gld-1, used for rescue of C. elegans q485 homozygotes

Depositor

Eric Haag

Article

Beadell et al Proc Natl Acad Sci U S A. 2011 Dec 6;108

Insert

gld-1 (Cbr-gld-1 Caenorhabditis briggsae)

Tags

FLAG and His

Expression

Worm

Promoter

gld1

Available Since

Feb. 25, 2015

Availability

Academic Institutions and Nonprofits only
pCMB-ERr

Plasmid

#61171

Purpose

Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtWAK2-mCherry-HDEL

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pET:CACT:GFP

Plasmid

#60737

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHJ33

Plasmid

#48362

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-34

Plasmid

#46652

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-34

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pX21

Plasmid

#46849

Purpose

Gene targeting to generate Trp53 Platform (PLF) mouse

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

129/Ola mouse Trp53 gene, Puro and attP sites

Use

Mouse Targeting

Mutation

attP flanked PGK-Neo

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-60

Plasmid

#46658

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-60

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-59

Plasmid

#46657

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-59

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-46

Plasmid

#46655

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-46

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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