We narrowed to 19,362 results for: gateway

Showing: 11621 - 11640 of 19362 results

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actc1b-2PH-PLCd-mKate2

Plasmid

#109500

Purpose

Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-2PH-PLCd-mKate2

Use

Zebrafish plasmids

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E p2a-tomato

Plasmid

#135211

Purpose

Red fluorescent tag

Depositor

David Tobin

Publication

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

tdTomato

Use

Synthetic Biology

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_TetOn_Hu_HEC1(WT)

Plasmid

#136353

Purpose

Lentiviral inducible expression (TetOn) of human HEC1

Depositor

Guillermo de Cárcer

Insert

HEC1 (NDC80 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME DN-E-cadherin

Plasmid

#135209

Purpose

Dominate Negative zebrafish e-cadherin label

Depositor

David Tobin

Publication

Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

Insert

CDH1

Use

Synthetic Biology

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pME tdTomato-NLS

Plasmid

#135205

Purpose

Red fluorescent tag

Depositor

David Tobin

Insert

tdTomato

Use

Synthetic Biology

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-Kaede

Plasmid

#132976

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

Depositor

Nathan Lawson

Publication

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

Kaede
gata2a endothelial enhancer (x6) and a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Publication

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Gateway Cloning and Synthetic Biology

Tags

Synthetic Intron and mGFP

Expression

Worm

Promoter

Pcol-19

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Publication

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

Plasmid

#121835

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHO_pTEFmut7_CggR_ble

Plasmid

#124584

Purpose

Plasmid for genomic integration of CggR repressor regulated by the pTEFmut7 promoter

Depositor

Matthias Heinemann

Publication

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

cggR (cggR Synthetic)

Use

Synthetic Biology

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Control-cx43.4-48H-pPRISM

Plasmid

#117801

Purpose

Control to test technique on a target that is known to work

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAGHA-SCAF8

Plasmid

#122470

Purpose

Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression

Depositor

Jesper Svejstrup

Publication

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform C CRISPR resistant (SCAF8 Human)

Tags

FLAGHA

Expression

Mammalian

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV 3xFLAG-GABARAP G116

Plasmid

#123099

Purpose

Expresses 3xFLAG-GABARAP G116 in mammalian cells.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B2-GFP

Plasmid

#123109

Purpose

Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL1 G116A

Plasmid

#123101

Purpose

Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT2-5xERE:GFP

Plasmid

#125690

Purpose

Transgene to report estrogen receptor activity in zebrafish

Depositor

Daniel Gorelick, Marnie Halpern

Publication

Gorelick et al Endocrinology. 2011 Jul;152(7):2690-703.

Insert

5xERE:GFP

Use

Zebrafish expression

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Publication

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-GTF2IRD1__ALK

Plasmid

#116821

Purpose

Lentiviral expression of GTF2IRD1__ALK fusion

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GTF2IRD1__ALK

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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