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pSB819-PKR-hum-LFA

Plasmid

#20051

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01(ΔL2)

Plasmid

#80050

Purpose

Expression of EPN-01 mutated in ALIX binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaYP)

Tags

Myc

Expression

Mammalian

Mutation

YP37AA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Tags

Histidine Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01*(ΔL1)

Plasmid

#80042

Purpose

Expression of PTAP L domain mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

pCMV-EPN-01*(ΔL2)

Tags

Myc

Expression

Mammalian

Mutation

PTAP sequence in p6 mutated to Ala

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL2)

Plasmid

#80043

Purpose

Expression of YPxL L domain mutantd EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Mutation

YP37 in p6 mutated to AA

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum-LYS

Plasmid

#20047

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

F489Y

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-FFS

Plasmid

#20045

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

L394F

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pWormgate2-myo-3p-mCherry-P2A-flag-UltraID

Plasmid

#227174

Purpose

For organ-specific expression of separated mCherry and UltraID in the body-wall muscle and neighboring vulval muscle cells of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

mCherry::P2A::UltraID

Expression

Worm

Promoter

myo-3

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-3p-gfp-halo

Plasmid

#227171

Purpose

For organ-specific expression of GFP-Halo in the body-wall muscle and neighboring vulval muscle cells of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

GFP

Tags

Halotag

Expression

Worm

Promoter

myo-3

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-2p-mCherry-P2A-flag-UltraID

Plasmid

#227172

Purpose

For organ-specific expression of separated mCherry and UltraID in the pharyngeal muscle of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

mCherry::P2A::UltraID

Expression

Worm

Promoter

myo-2

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-ges-1p-mCherry-P2A-flag-UltraID

Plasmid

#227173

Purpose

For organ-specific expression of separated mCherry and UltraID in the intestine of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

mCherry::P2A::UltraID

Expression

Worm

Promoter

ges-1

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_JUNO_GFPnes

Plasmid

#240499

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

JUNO (Izumo1r Mouse)

Tags

GFPnes and V5

Expression

Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13F4C

Plasmid

#178023

Purpose

E. coli - B. subtilis nicking vector pProUSER13F4C. Ap-BBR1; ΔsigF-insertion-site; Km; manR-PmanA

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Pman

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13E3F

Plasmid

#178026

Purpose

E. coli - B. subtilis nicking vector pProUSER13E3F. Ap-BBR1; yhgE-insertion-site; Spc; tetR-Ptet

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Ptet

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13G2F

Plasmid

#178027

Purpose

E. coli - B. subtilis nicking vector pProUSER13G2F . Ap-BBR1; rsbP-insertion-site; Ery; tetR-Ptet

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Ptet

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-RBAP46/RBBP7

Plasmid

#109210

Purpose

Encodes human full-length RBBP7 (aka RBAP46) to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length RBBP7 (RBBP7 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_AfRbcLS

Plasmid

#229518

Purpose

Expresses Anthoceros fusiformis Rubisco large and small subunit (C terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco large subunit
Rubisco small subunit

Tags

hexahistidine

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_PcRbcLS

Plasmid

#229519

Purpose

Expresses Phaeoceros carolinianus Rubisco large and small subunit (C terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco large subunit
Rubisco small subunit

Tags

hexahistidine

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_NoRbcLS

Plasmid

#229520

Purpose

Expresses Notothylas orbicularis Rubisco large and small subunit (C terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco large subunit
Rubisco small subunit

Tags

hexahistidine

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_PpRbcLS

Plasmid

#229521

Purpose

Expresses Phymatoceros phymatodes Rubisco large and small subunit (C terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco large subunit
Rubisco small subunit

Tags

hexahistidine

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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