We narrowed to 16,279 results for: LEA
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Plasmid#122971PurposeReporter of botulinum toxin B cleavageDepositorInsertEGFP-VAMP3-GST
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP mCherry-ApoB-EGFP
Plasmid#112859PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFPDepositorInsertmCherry-apoB-EGFP
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRExpressionPlantAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb
Plasmid#182009PurposeCMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell linesDepositorInsertIgKchL_HA_STf_myc_PDGFRtmb
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046979
Plasmid#145611PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
HYG-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193315PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
Tags4FLAGExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mt-ro1GFP
Plasmid#82407PurposeExpression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cellsDepositorInsertro1GFP
Tagsleader sequence of the E1α subunit of pyruvate de…ExpressionMammalianMutationC48S/F64L/T65S/S147C/Q204CPromoterCMVAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
Tags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AMPKA2
Plasmid#36194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAMPKA2 (PRKAA2 Human)
TagsHis tag with thrombin cleavageExpressionBacterialMutationcontains KINASE DOMAIN onlyAvailable SinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046920
Plasmid#145717PurposeBacterial Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-SMVP-Cas9N-U6-gRNA M3
Plasmid#80936PurposeExpresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEL1284
Plasmid#175960PurposeFor expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
Tags6xHis 2xQH and TEV cleavage site on LetAExpressionBacterialPromoteraraBADAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mVenus-hCOL1A1
Plasmid#140111PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
TagsmVenusExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7653_v2
Plasmid#126855Purpose7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffoldDepositorInsertII_CS3_7653_v2
UseSynthetic BiologyAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_NES_C1
Plasmid#85061PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (1-575)
Plasmid#1318DepositorInsertHsp104 (1-575) (HSP104 Budding Yeast)
Tags6X His + TEV cleavageExpressionBacterialMutationaa1-575 of Hsp104Available SinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-NLS-Smad2
Plasmid#14931DepositorInsertNLS-Smad2 (SMAD2 Human)
TagsFlagExpressionMammalianMutationfull-length Smad2 is fused to a nuclear-localizat…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
TagsNLS (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-UBC9-K14R-A129K
Plasmid#228948Purposeexpress a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)DepositorInsertUBC9 (UBE2I Human)
TagsHis tag and thrombine cleavage site : MGHHHHHHSSG…ExpressionBacterialMutationchanged Lysine 14 to Arginine, changed Alanine 12…PromoterT7-lacAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only