We narrowed to 4,385 results for: 187

1. Cas-Acr (wtAcrIIA4)

   Plasmid

   #128115

   Purpose

   Expresses SpyCas9 fused to wild-type AcrIIA4

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   CascAID (wtAcrIIA4)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA EMX1

   Plasmid

   #128121

   Purpose

   Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA EMX1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSuperRetro mouse Hic-5

   Plasmid

   #11187

   Depositor

   Bruce Spiegelman

   Article

   Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

   Insert

   Hic-5 shRNA (Tgfb1i1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pFOSGE_CUS1E04

   Plasmid

   #71076

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Sirt4 (Sirt4 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pc3XB-ZF137

   Plasmid

   #13187

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   ZF137
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest17_Gcn4 solvvol W

   Plasmid

   #231878

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest17_Gcn4 solvvol

   Plasmid

   #231877

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest17_Gcn4 ILVtoED W

   Plasmid

   #231871

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lvl0-G-Gal4-VPR

   Plasmid

   #229187

   Purpose

   lvl 0 5 gene

   Depositor

   Ron Weiss

   Article

   Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

   Insert

   Gal4-VPR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCF.111

    Plasmid

    #227187

    Purpose

    pORTMAGE-Ec1 + supplement E. coli SSB

    Depositor

    Seth Shipman

    Article

    Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

    Insert

    CspRecT, mutL_E32K, E. coli SSB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutL E32K

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GEARBOCS-Vamp2-GeneTRAP

    Plasmid

    #218187

    Purpose

    To KO and genetrap mouse Vamp2 simultaneously

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Vamp2 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-C1-BBS18

    Plasmid

    #218718

    Purpose

    Expresses N-terminally EGFP-tagged BBS18 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS18
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-MARK4(v3)

    Plasmid

    #166187

    Purpose

    Gateway entry vector for N. furzeri MARK4

    Depositor

    Daniel Jarosz

    Article

    Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

    Insert

    MARK4(v3)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-ConVERGD-eGFP-W3SL

    Plasmid

    #218748

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRegev_ExonSkip_Backbone

    Plasmid

    #204187

    Purpose

    Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    First exon and intron for beta globin splicing minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SITS-ccdb-mCherry

    Plasmid

    #118753

    Purpose

    Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ccdb
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SITS-ccdb-EGFP

    Plasmid

    #118752

    Purpose

    Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ccdb
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDest-624 (attR4-attR2)

    Plasmid

    #161879

    Purpose

    Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDK134

    Plasmid

    #134187

    Purpose

    Expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 aa 69 - 479 (knl-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA HBB

    Plasmid

    #128120

    Purpose

    Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA HBB + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge