We narrowed to 4,125 results for: 272

1. Met133ILeu, Met186ILeu ELL2

   Plasmid

   #127264

   Purpose

   For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Two Mets (133,186 )to ILeu Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Met331Leu ELL2

   Plasmid

   #127262

   Purpose

   For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met331 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Met331 to ILeu Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. COOHend ELL2

   Plasmid

   #127255

   Purpose

   Expresses the C terminal segment of human ELL2 in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (C-terminal end) (ELL2 Human)
   

   Use

   Other

   Tags

   Expression

   Mammalian

   Mutation

   contains only the last 389 aa

   Promoter

   pEF1a

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Met1ILeu ELL2

   Plasmid

   #127258

   Purpose

   For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met1 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Met1 to Ileu by Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ELL2mRNA+polyA

   Plasmid

   #127256

   Purpose

   For in vitro translation of human ELL2 with 30 nt pA tail

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   shortens 3'UTR to 40 bp adds A30 tail

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NH2end ELL2

   Plasmid

   #127252

   Purpose

   Expresses the N terminal segment of human ELL2 in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (1-287) (ELL2 Human)
   

   Use

   Other

   Tags

   His6X

   Expression

   Mammalian

   Mutation

   contains only amino acids 1-289

   Promoter

   pEF1a

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA tagged Xba ELL2

   Plasmid

   #127268

   Purpose

   For in vitro translation of WT ELL2 with HA tag inserted near N-term

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

   Promoter

   T7

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAN1646

    Plasmid

    #127205

    Purpose

    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-hs-HMGA2

    Plasmid

    #52727

    Purpose

    retroviral expression of human HMGA2

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    HMGA2 (HMGA2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100854

    Purpose

    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100853

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV Retrograde and AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT-TO-NOD2-EGFP

    Plasmid

    #131207

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD2 (NOD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. nAChR beta2 WT

    Plasmid

    #24272

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR beta2 WT (Chrnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p2T-U6-sgGAA

    Plasmid

    #232724

    Purpose

    Cas9 guide RNA targeting GAA repeats for A-to-G base editing

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    SpCas9 gRNA targeting GAA repeats
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-tetO-Ets2

    Plasmid

    #70272

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Ets2 (Ets2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge