Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
PD-1 and BTLA regulate T cell signaling differentially and only partially through SHP1 and SHP2.
Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma Y, Wang H, Bennett EJ, Fu G, Hui E
J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 10.1083/jcb.201905085. PubMed Article

Plasmids from Article

ID Plasmid Purpose
180790pHR-PD-1 (Y223F, Y248F)-mGFPExpression of human PD-1 (Y223F, Y248F) fused to mGFP
180791pHR-BTLA-mGFPExpression of human BTLA fused to mGFP
180792pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFPExpression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mGFP
180794pHR-PD-1ecto&tmd-BTLAicd-mGFPExpression of human PD-1 extracellular domain and transmembrane domain fused to BTLA intracellular domain followed by mGFP
180795pHR-SV40-BTLAecto&tmd-PD-1icd-mGFPExpression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mGFP
180798pMIG II-BTLA-mGFPExpression of mouse BTLA fused to mGFP
180799pMIG II SIN-SV40-mCherry-SHP1Expression of mouse SHP1 fused to N-terminal mCherry
180800pMIG II SIN-SV40-mCherry-SHP2Expression of mouse SHP2 fused to N-terminal mCherry
180805pET28-His10-BTLAicd (AA190-289)-TwinStrepExpression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep
180806pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrepExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
180807pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrepExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep
180808pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrepExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep
180809pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrepExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep
180811pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrepExpression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep
180812pET28a-His10-SHP1_PTPase (243-541aa)Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)
180814pGEX-6p-2-GST-PD-1icd (AA194-288)Expression of GST fused human PD-1 intracellular domain (AA194-288)
180815pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)Expression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)

Antibodies from Article