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pDK480

Plasmid

#134197

Purpose

Mammalian expression of Flip in SKAP and IRES GFP

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)

Tags

IRES-GFP

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5HA-MAGE-KA240,245

Plasmid

#89140

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429 with KA240,KA245 ubiquitination sites mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Tags

HA

Expression

Mammalian

Mutation

K240A, K245A

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFL-TK-AID3'UTR-MUT

Plasmid

#19987

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Mutation

Mutation

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pet 3d double tentacle delete CP

Plasmid

#13301

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)

Expression

Bacterial

Mutation

Capping protein alpha 1 (with 28 amino acids dele…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
3187 pSFFV-neo Bad cDNA ORF

Plasmid

#8756

Depositor

Stanley Korsmeyer

Article

Zha et al Cell 1996 Nov 15;87(4):619-28.

Insert

BAD (Bad Mouse)

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pEN_TT PCSK5(sgRR,M452I)-V5

Plasmid

#232450

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_M452I mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c; M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR,T288P)-V5

Plasmid

#232451

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_T288P mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(T288P) (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c; T288P

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR)-V5 hygro

Plasmid

#232452

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR,M452I)-V5 hygro

Plasmid

#232453

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5_M452I mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c; M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR,T288P)-V5 hygro

Plasmid

#232454

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5_T288P mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(T288P) (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c; T288P

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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LSM044-2 CMV-TO-CCR7-V2-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233396

Purpose

CCR7-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR7-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

Plasmid

#233443

Purpose

PiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Plasmid

#233446

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-PCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Plasmid

#233442

Purpose

PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

Plasmid

#233441

Purpose

PiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Plasmid

#233444

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-MCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

Plasmid

#233413

Purpose

LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ054 (PB)SFFV-LIDARsensor (hygR)

Plasmid

#233419

Purpose

PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)

Plasmid

#233430

Purpose

LIDAR all components on single transcript (as shown in Figure 4A), using EMCV IRES to separate each component

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

sensor-IRESv0-LIDARst(IRESv0-AVPR2)

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM

Plasmid

#233429

Purpose

Positive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-LIDARsensor(W)-hIgG1-TM

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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