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pAc5.1B-lambdaN-HA-DmNot1_1147-2505_I

Plasmid

#146615

Purpose

Insect Expression of DmNot1_1147-2505

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-2505 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent and one non silent mutation G1999S compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2_1-401_I

Plasmid

#146616

Purpose

Insect Expression of DmNot2_1-401

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_1-401 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation C267T compared to the ORF ext…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCR4_168-552_I

Plasmid

#146603

Purpose

Insect Expression of DmCCR4_168-552

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552 (twin Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1147-1717_I

Plasmid

#146605

Purpose

Insect Expression of DmNot1_1147-1717

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-1717 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

2 non silent mutations compared to the sequence g…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1147-2505_I

Plasmid

#146606

Purpose

Insect Expression of DmNot1_1147-2505

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-2505 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent and one non silent mutation G1999S compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2_1-401_I

Plasmid

#146607

Purpose

Insect Expression of DmNot2_1-401

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_1-401 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation C267T compared to the ORF ext…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEE057

Plasmid

#176814

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

608

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-As

Plasmid

#138103

Purpose

Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-As

Plasmid

#138097

Purpose

Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-As

Plasmid

#138100

Purpose

Golden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1408

Plasmid

#99032

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1928

Plasmid

#99033

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

Plasmid

#141395

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

eGFP

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PPP1R12A wt

Plasmid

#31658

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-PPP1R12A wt

Plasmid

#31669

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pDiCre-mCherry

Plasmid

#164572

Purpose

Integration of a DiCre cassette in PbGFP parasite genome

Depositor

Olivier Silvie

Article

Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

Inserts

FKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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