We narrowed to 24,194 results for: Spr

1. pCas-mut4_1_2_VA

   Plasmid

   #186441

   Purpose

   Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas-4/1_2_VB

   Plasmid

   #186442

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas-4/1Δ2_VB

   Plasmid

   #186443

   Purpose

   Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas12(PI)

   Plasmid

   #186448

   Purpose

   Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12a (PI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K613A, K617A

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas12b(PI)

   Plasmid

   #186451

   Purpose

   Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b (PI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R122A, G143P

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCas-mut4/1_2_VB

   Plasmid

   #186444

   Purpose

   Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas4 domain (K81A)

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas_4(I-U)/1_2_VB

   Plasmid

   #186445

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas4 domain swapped with Cas4 domain from G. sulf…

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAIP4_1TJ6

   Plasmid

   #234167

   Purpose

   Heterologous protein expression of sprouty-related, EVH1 domain-containing protein 1 (Spred-1) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1TJ6 (spred1 Xenopus tropicalis)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. PAJ389

   Plasmid

   #104147

   Purpose

   dCas9 plasmid expressing 2A sRNA trigger

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PAJ390

    Plasmid

    #104148

    Purpose

    dCas9 plasmid expressing 3A sRNA trigger

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEM-eCas9

    Plasmid

    #154925

    Purpose

    aTc inducible eCas9

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    eCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    pTet

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEM-OptiCas9

    Plasmid

    #154927

    Purpose

    aTc inducible OptiCas9

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    OptiCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    R661A, K1003H

    Promoter

    pTet

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEM-HypaCas9

    Plasmid

    #154929

    Purpose

    aTc inducible HypaCas9

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    HypaCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    N692A/M694A/Q695A/H698A

    Promoter

    pTet

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PAJ379

    Plasmid

    #104143

    Purpose

    Expresses 3A igRNA and sfGFP

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PAJ407

    Plasmid

    #104144

    Purpose

    Expresses 3A_v9 igRNA and sfGFP

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PAJ426

    Plasmid

    #104145

    Purpose

    Expresses HIV3 igRNA and sfGFP

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PAJ469

    Plasmid

    #104146

    Purpose

    Expresses 3A igRNA and Hybrid-promoter sfGFP

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSL1045

    Plasmid

    #129525

    Purpose

    pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    dSpCas9 (Other)
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PAJ408

    Plasmid

    #104150

    Purpose

    dCas9 plasmid expressing full length mKate2 mRNA without RBS

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEM-eCas9-D1135E

    Plasmid

    #154926

    Purpose

    aTc inducible eCas9 with D1135E mutation

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    eCas9-D1135E
    

    Use

    CRISPR and Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, D1135E

    Promoter

    pTet

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge