We narrowed to 3,093 results for: try
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Plasmid#84968PurposeType 3a, α-Amylase followed by αMFΔ - secretion signalDepositorInsertα-Amylase followed by MFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK018-3b-RFP_Sec
Plasmid#84977PurposeType 3b, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
phpBCL2-FL(pA)
Plasmid#42593DepositorInserthairpin - BCL2 5'UTR - firefly luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-FW/AA
Plasmid#12293DepositorInsertTLR2 (Tlr2 Mouse)
TagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y, F774A and W775A; signal peptide (aa1…Available sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)
Plasmid#139445PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
phpRL-BCL2-FL-pA
Plasmid#42595DepositorInserthairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHJ41
Plasmid#48360PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAcUW51-gE2
Plasmid#11621DepositorInsertHSV-1 glycoprotein E
TagsHisExpressionInsectMutationdeleted amino acids 391-550Available sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-act1p
Plasmid#120233PurposeCarries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing act1p promoter and apramycin resistance gene
UseSynthetic BiologyMutationaac(3)IV (apramycin resistance gene)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-CTRLg1
Plasmid#139450PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-Neo-CTRLg2
Plasmid#139451PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC
Plasmid#114376Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
UseAAVTagsEYFP, Kv2.1C, and TlcnCExpressionMammalianPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorPublicationInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHJ22
Plasmid#48358PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi
Plasmid#89740PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11DepositorInsertNLS-OptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ145
Plasmid#98405PurposeDonor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ149
Plasmid#98407PurposeDonor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianPromoterCMVAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only