|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||33345||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4922
Vector typeTrypanosoma brucei expression vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namecre recombinase
Insert Size (bp)1032
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byJohn Donelson, Dept. Biochemistry, University of Iowa
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid is also known as pLEW100creTS.
Cre recombinase is flanked by UTR sequences to allow regulated T7 promoter-driven low level expression after cleavage by NotI and integration into a rRNA locus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLEW100cre-EP1 was a gift from George Cross (Addgene plasmid # 33345 ; http://n2t.net/addgene:33345 ; RRID:Addgene_33345)
For your References section:CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei. Scahill MD, Pastar I, Cross GA. Mol Biochem Parasitol. 2008 Jan;157(1):73-82. Epub 2007 Oct 6. 10.1016/j.molbiopara.2007.10.003 PubMed 18006158