PurposeTargets TbMCM-BP for Cre-lox mediated knockout in T. brucei
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48355||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerCross Lab
- Total vector size (bp) 7015
Vector typeCre/Lox ; Knockout in T. Brucei
Selectable markersHygromycin ; Gancyclovir
Growth in Bacteria
Gene/Insert nameloxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies
- Cloning method Unknown
- 5′ sequencing primer tb#44 CTGGTTAGTATGGACTTCTCTAGA
- 3′ sequencing primer pBRrevBam (Common Sequencing Primers)
For additional information, see http://tryps.rockefeller.edu/trypsru2_cre-lox.html
Addgene's sequence with primer Hygro-R shows several mismatches within the TbMCM-BP 5' homology arm as well as other small changes compared to the depositor's reference sequence. These changes should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSY45 was a gift from George Cross (Addgene plasmid # 48355 ; http://n2t.net/addgene:48355 ; RRID:Addgene_48355)
For your References section:Strategies to construct null and conditional null Trypanosoma brucei mutants using Cre-recombinase and loxP. Kim HS, Li Z, Boothroyd C, Cross GA. Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 10.1016/j.molbiopara.2013.08.001. 10.1016/j.molbiopara.2013.08.001 PubMed 23954366