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(Plasmid #33344)


Item Catalog # Description Quantity Price (USD)
Plasmid 33344 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4922
  • Vector type
    Trypanosoma brucei expression vector
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    cre recombinase
  • Insert Size (bp)
  • GenBank ID

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    John Donelson, Dept. Biochemistry, University of Iowa

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is also known as pLEW100creTS-SAS.

Cre recombinase is flanked by UTR sequences to allow regulated T7 promoter-driven low level expression after cleavage by NotI and integration into a rRNA locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEW100cre-EP1-6G was a gift from George Cross (Addgene plasmid # 33344 ; ; RRID:Addgene_33344)
  • For your References section:

    CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei. Scahill MD, Pastar I, Cross GA. Mol Biochem Parasitol. 2008 Jan;157(1):73-82. Epub 2007 Oct 6. 10.1016/j.molbiopara.2007.10.003 PubMed 18006158