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pDONR223_RIT1_p.R122L

Plasmid

#82737

Purpose

Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RIT1 (RIT1 Human)

Use

Gateway entry vector

Mutation

R122L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKR1B1_p.F252L

Plasmid

#82743

Purpose

Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AKR1B1 (AKR1B1 Human)

Use

Gateway entry vector

Mutation

F252L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STC2_p.R5W

Plasmid

#82744

Purpose

Gateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway entry vector

Mutation

R5W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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LRGUK gRNA (BRDN0001147114)

Plasmid

#78085

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRGUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRGUK (LRGUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK1G1 gRNA (BRDN0001146475)

Plasmid

#77441

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1G1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1G1 (CSNK1G1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHA7 gRNA (BRDN0001146678)

Plasmid

#77353

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHA7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHA7 (EPHA7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K9 gRNA (BRDN0001145380)

Plasmid

#77308

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K9

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K9 (MAP3K9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FER gRNA (BRDN0001148090)

Plasmid

#76800

Purpose

3rd generation lentiviral gRNA plasmid targeting human FER

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FER (FER Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FER gRNA (BRDN0001145815)

Plasmid

#76801

Purpose

3rd generation lentiviral gRNA plasmid targeting human FER

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FER (FER Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HIPK2 gRNA (BRDN0001162198)

Plasmid

#76751

Purpose

3rd generation lentiviral gRNA plasmid targeting human HIPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HIPK2 (HIPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAK gRNA (BRDN0001148291)

Plasmid

#76709

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAK (MAK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GTF2H1 gRNA (BRDN0001146791)

Plasmid

#76598

Purpose

3rd generation lentiviral gRNA plasmid targeting human GTF2H1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GTF2H1 (GTF2H1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FRK gRNA (BRDN0001144749)

Plasmid

#76240

Purpose

3rd generation lentiviral gRNA plasmid targeting human FRK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FRK (FRK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ANKK1 gRNA (BRDN0001146770)

Plasmid

#76037

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANKK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ANKK1 (ANKK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK3 gRNA (BRDN0001145644)

Plasmid

#75976

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK3 (STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CMPK2 gRNA (BRDN0001145007)

Plasmid

#75805

Purpose

3rd generation lentiviral gRNA plasmid targeting human CMPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CMPK2 (CMPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3R4 gRNA (BRDN0001148885)

Plasmid

#75594

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3R4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3R4 (PIK3R4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3R4 gRNA (BRDN0001148908)

Plasmid

#75595

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3R4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3R4 (PIK3R4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARD14-L156P

Plasmid

#69918

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

L156P

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
CMV-10-3xFLAG-LGP2-MIIa

Plasmid

#58684

Purpose

Expresses LGP2 with a mutation to motif IIa in mammalian cells

Depositor

Curt Horvath

Article

Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

Insert

LGP2-MIIa (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

mutation to Motif IIA, K138E and Y142F

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only